The base-pairing specificity of cellulose-pdT9.
The base-pairing specificity of cellulose-pdT9.
复制标题
纤维素-pdT9 的碱基配对特异性。
作者：
S. Gillam;K. Waterman;M. Smith
通过热洗脱研究了含有单核苷酸取代的寡腺苷酸与纤维素-pdT9 相互作用的稳定性。就寡脱氧核糖腺苷酸而言，内部 dA 被 dC、dG 或 dT 取代会导致不稳定。就寡核糖腺苷酸而言，用 C 或 U 替换内部 A 残基可显着减少不稳定。结果表明，在将脱氧核糖多核苷酸-脱氧核糖多核苷酸杂交的特异性数据外推至脱氧核糖多核苷酸-核糖多核苷酸类型的结构时应谨慎。
The stability of the interaction of oligoadenylates, containing single nucleotide substitutions, with cellulose-pdT9 has been studied by thermal elution. In the case of oligodeoxyriboadenylates, the replacement of an internal dA by dC, dG or dT caused destabilization. In the case of oligoriboadenylates, replacement of an internal A residue by C or U resulted in a significantly lesser destabilization. The results indicate that caution should be exercised in extrapolation of data on the specificity of deoxyribonpolynucleotide-deoxyribopolynucleotide hybridization to structure of the type deoxyribopolynucleotide-ribopolynucleotide.