Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein
Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein
复制标题
单纯疱疹病毒 1 型主要 DNA 结合蛋白与核酸的相互作用以及对病毒 DNA 聚合酶的刺激
DOI：
10.1128/jvi.52.3.727-733.1984
复制
发表时间：
1984
影响因子：
5.4
通讯作者：
A. C. Weir
中科院分区：
文献类型：
--
作者：
W. Ruyechan;A. C. Weir
通过过滤器结合试验和电子显微镜检查了1型单纯疱疹病毒（HSV-1）主要DNA结合蛋白、感染细胞多肽8（ICP 8）与核酸的相互作用。在宽pH范围内进行的过滤器结合试验表明，蛋白质-DNA相互作用的最佳pH约为7.6。热灭活研究表明，ICP 8在高达40 ℃的温度下是稳定的，在45 ℃及以上孵育时结合活性迅速丧失。竞争结合实验已经建立了ICP 8对以下核酸的以下相对亲和力：与噬菌体fd DNA一致的单链HSV-1 DNA大于多聚核糖腺苷酸，远大于与d（pCpT）5一致的双链HSV-1 DNA。负染色的ICP 8-单链DNA复合物的观察表明，ICP 8以规则的重复方式沿DNA的长度沿着结合。这些复合物的平均宽度为9.3 +/- 0.8 nm。终于来了最后，将纯化的ICP 8加入HSV-1 DNA聚合酶反应导致病毒聚合酶活性的刺激。
The interaction of the herpes simplex virus type 1 (HSV-1) major DNA-binding protein, infected-cell polypeptide 8 (ICP8), with nucleic acids has been examined by a filter-binding assay and electron microscopy. Filter-binding assays done over a broad pH range indicated that the optimum pH for the protein-DNA interaction is approximately 7.6. Heat inactivation studies showed that ICP8 is stable at temperatures up to 40 degrees C with a rapid loss of binding activity on incubation at 45 degrees C and above. Competition binding experiments have established the following relative affinities of ICP8 for the following nucleic acids: single-stranded HSV-1 DNA congruent to bacteriophage fd DNA greater than polyriboadenylate much greater than double-stranded HSV-1 DNA congruent to d(pCpT)5. Observation of negatively stained ICP8-single-stranded DNA complexes indicated that ICP8 binds along the length of the DNA in a regular repeating fashion. The average width of these complexes is 9.3 +/- 0.8 nms. Finally. Finally, addition of purified ICP8 to HSV-1 DNA polymerase reactions resulted in a stimulation of the viral polymerase activity.
DOI：
10.1073/pnas.79.19.5803
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHRYSOGELOS, S;GRIFFITH, J
通讯作者：
GRIFFITH, J