The Choice of Translation Initiation Site of the Rep Proteins from Goose Parvovirus P9-Generated mRNA Is Governed by Splicing and the Nature of the Excised Intron
The Choice of Translation Initiation Site of the Rep Proteins from Goose Parvovirus P9-Generated mRNA Is Governed by Splicing and the Nature of the Excised Intron
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DOI：
10.1128/jvi.01255-09
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发表时间：
2009-10-01
影响因子：
5.4
通讯作者：
Pintel, David J.
中科院分区：
文献类型：
--
作者：
Li, Long;Qiu, Jianming;Pintel, David J.
鹅细小病毒(GPV)Rep 1和Rep 2蛋白分别由P9产生的未剪接或在rep基因区内拼接的mRNA所编码。这些mRNAs以大致相等的比例存在。Rep 1的翻译从第一个8月开始，在未剪接的P9产生的RNA中；然而，在剪接的P9产生的RNA中绕过了这个8月，而Rep 2的翻译主要在下一个起始的8月下游开始。我们发现，GPV Rep编码mRNAs翻译起始位置的选择既受剪接过程本身的控制，也受切除内含子的性质控制。
The goose parvovirus (GPV) Rep 1 and Rep 2 proteins are encoded by P9-generated mRNAs that are either unspliced or spliced within the rep gene region, respectively. These mRNAs are present in an approximately equal ratio. The translation of Rep 1 was initiated from the first AUG in unspliced P9-generated mRNA; however, this AUG was bypassed in spliced P9-generated RNA and Rep 2 translation initiated predominately at the next initiating AUG downstream. We show that the choice of the site of initiation of translation of GPV Rep-encoding mRNAs is governed both by the splicing process itself and by the nature of the excised intron.