Detailed analysis of leucokinin-expressing neurons and their candidate functions in the Drosophila nervous system
Detailed analysis of leucokinin-expressing neurons and their candidate functions in the Drosophila nervous system
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DOI：
10.1007/s00441-009-0890-y
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发表时间：
2010-02-01
影响因子：
3.6
通讯作者：
Herrero, Pilar
中科院分区：
文献类型：
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作者：
de Haro, Maria;Al-Ramahi, Ismael;Herrero, Pilar
白细胞激肽（leucokinin，LK）神经元在果蝇中枢神经系统（Drosophilamelanogaster，CNS）中的分布已被免疫标记所描述。然而，没有详细的基础信息的输入或输出连接的神经突，然后可用。在这里，我们提供了一个更准确的形态学描述，采用一种新的LK特异性GAL 4线，概括LK的表达。为了分析LK神经元的可能的传入和传出神经候选者，我们将该lk-GAL 4系与其他CNS-Gal 4系一起使用，与针对各种神经肽或神经递质的抗血清组合。我们在大脑中发现了四种LK神经元。(1)侧角神经元连接触角小球和蘑菇体。(2)食管下神经元将味觉感受器连接到食管下神经节和腹神经索。(3)前部神经元支配环腺的心脏体，但令人惊讶的是，从第三龄起在这些神经元中检测不到LK表达。(4)一组腹神经节神经元连接到幼虫的背正中束，并将其轴突发送到节段肌肉8。因此，在我们的研究中采用的方法可用于识别单个神经肽能神经元，从而表征其分化的功能线索或发育转化。
The distribution of leucokinin (LK) neurons in the central nervous system (CNS) of Drosophila melanogaster was described by immunolabelling many years ago. However, no detailed underlying information of the input or output connections of their neurites was then available. Here, we provide a more accurate morphological description by employing a novel LK-specific GAL4 line that recapitulates LK expression. In order to analyse the possible afferent and efferent neural candidates of LK neurons, we used this lk-GAL4 line together with other CNS-Gal4 lines, combined with antisera against various neuropeptides or neurotransmitters. We found four kinds of LK neurons in the brain. (1) The lateral horn neurons connect the antennal glomerula to the mushroom bodies. (2) The suboesophageal neurons connect the gustatory receptors to the suboesophageal ganglia and ventral nerve cord. (3) The anterior neurons innervate the corpus cardiacum of the ring gland but LK expression is surprisingly not detectable from the third instar onwards in these neurons. (4) A set of abdominal ganglion neurons connect to the dorsal median tract in larvae and send their axons to a segmental muscle 8. Thus, the methods employed in our study can be used to identify individual neuropeptidergic neurons and thereby characterize functional cues or developmental transformations in their differentiation.