Genome-Based Analysis of Heme Biosynthesis and Uptake in Prokaryotic Systems
Genome-Based Analysis of Heme Biosynthesis and Uptake in Prokaryotic Systems
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DOI：
10.1021/pr8004309
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发表时间：
2008-11-01
影响因子：
4.4
通讯作者：
Rosato, Antonio
中科院分区：
文献类型：
--
作者：
Cavallaro, Gabriele;Decaria, Leonardo;Rosato, Antonio
血红素是许多蛋白质的修饰性基团，执行各种关键的生物学功能。此外，对于许多病原生物来说，血红素(从宿主获得)可能是铁的一个非常重要的来源。生物体可以通过从外部来源吸收，或通过专门的生物合成途径产生辅因子，或两者兼而有之，以满足其对血红素的需求。在这里，我们分析了474种原核生物中特定参与血红素生物合成和吸收过程的蛋白质的分布。这些数据使我们能够根据与已知系统的相似性来识别哪些生物体能够不执行、一个或两个都执行过程。挑出了一些沿着通路的一个或多个蛋白质具有不寻常修饰的特定实例。对于参与血红素吸收的两个关键蛋白结构域，我们可以建立一系列结构模型，这些模型提出了可能的血红素结合的替代模式。给出了今后实验工作的方向。
Heme is the prosthetic group of many proteins that carry out a variety of key biological functions. In addition, for many pathogenic organisms, heme (acquired from the host) may constitute a very important source of iron. Organisms can meet their heme demands by taking it up from external sources, by producing the cofactor through a dedicated biosynthetic pathway, or both. Here we analyzed the distribution of proteins specifically involved in the processes of heme biosynthesis and heme uptake in 474 prokaryotic organisms. These data allowed us to identify which organisms are capable of performing none, one, or both processes, based on the similarity to known systems. Some specific instances where one or more proteins along the pathways had unusual modifications were singled out. For two key protein domains involved in heme uptake, we could build a series of structural models, which suggested possible alternative modes of heme binding. Future directions for experimental work are given.