Effects of dietary glycyl-glutamine on growth performance, small intestinal integrity, and immune responses of weaning piglets challenged with lipopolysaccharide
Effects of dietary glycyl-glutamine on growth performance, small intestinal integrity, and immune responses of weaning piglets challenged with lipopolysaccharide
复制标题
日粮甘氨酰谷氨酰胺对脂多糖攻击的断奶仔猪生长性能、小肠完整性和免疫反应的影响
DOI：
10.2527/jas.2008-1120
复制
发表时间：
2009-12-01
影响因子：
3.3
通讯作者：
Zou, S. T.
中科院分区：
文献类型：
--
作者：
Jiang, Z. Y.;Sun, L. H.;Zou, S. T.
本研究旨在探讨饲粮中添加甘氨酰谷氨酰胺（Gly-Gln）对应激和非应激仔猪断奶后生长、小肠形态和免疫功能的影响。将猪（n = 144;最初4.49 kg，14日龄）随机分配至环境控制的保育室中的24个围栏（6只猪/围栏），并以2 x 2析因处理安排（6个围栏/处理）分配至大肠杆菌脂多糖（LPS）攻击（PBS vs. LPS）和Gly-Gln补充（0 vs. 0.15%）。LPS为应激诱导剂，在21 d实验的第7 d和第14 d注射。从试验的第7天到第21天，LPS炎症刺激降低了仔猪的ADG（P < 0.05），并有降低ADFI（P = 0.06）的趋势。添加Gly-Gln可显著提高0 ~ 21日龄日增重和料肉比（P < 0.05）。在第21天（第二次LPS注射后1 wk），存在LPS攻击与饮食Gly-Gln的交互作用（P < 0.05），但难以确定Gly-Gln是否增加ADFI。对于ADG，观察到LPS攻击x饮食Gly-Gln相互作用的趋势（P = 0.07）。治疗组间淋巴细胞增殖无差异。在第14天（注射LPS后1 wk），LPS刺激增加了十二指肠隐窝深度（CD），降低了回肠绒毛高度（VH）与CD的比值（P < 0.05），而日粮中添加Gly-Gln则增加了回肠VH和十二指肠VH：CD（P < 0.05）。在实验期间，注射LPS使外周血IL-1 β浓度升高（P < 0.05），而饮食中添加Gly-Gln使外周血IL-1 β浓度降低（P < 0.05），但LPS攻击与饮食中添加Gly-Gln对IL-1 β浓度无交互作用。添加Gly-Gln的仔猪外周血IL-2浓度在第14天有升高趋势（P = 0.09），可溶性IL-2受体浓度在第7天有降低趋势（P = 0.06），因此，外周血IL-2/可溶性IL-2受体系统在试验的前2 wk有促进IL-2分泌的趋势。总之，相当大的抑制生长和免疫功能发生在早期断奶仔猪与LPS的挑战，这种抑郁症可以缓解饮食Gly-Gln补充独立的LPS挑战。
The objectives of this study were to determine the effects of dietary glycyl-glutamine (Gly-Gln) on postweaning growth, small intestinal morphology, and immune response of stressed or nonstressed piglets. Pigs (n = 144; initially 4.49 kg and 14 d of age) were randomly allocated to 24 pens (6 pigs/pen) in an environmentally controlled nursery and assigned to Escherichia coli lipopolysaccharide (LPS) challenge (PBS vs. LPS) and Gly-Gln supplementation (0 vs. 0.15%) in a 2 x 2 factorial arrangement of treatments with 6 pens/treatment. The LPS was the stress-inducing agent, and it was injected on d 7 and 14 of the 21-d experiment. Inflammatory challenge with LPS reduced ADG (P < 0.05) and tended to reduce ADFI (P = 0.06) of piglets from d 7 to 21 of the experiment. Supplementation of Gly-Gln increased ADG and G: F from d 0 to 21 (P < 0.05). On d 21 (1 wk after the second LPS injection), there was an LPS challenge x diet Gly-Gln interaction for ADFI (P < 0.05), but it was difficult to ascertain whether Gly-Gln increased ADFI. A trend for an LPS challenge x diet Gly-Gln interaction was observed for ADG (P = 0.07). There were no differences in lymphocyte proliferation among treatments. The LPS challenge increased crypt depth (CD) of the duodenum and decreased the ratio of villus height (VH) to CD of the ileum (P < 0.05) on d 14 (1 wk after the first LPS injection), whereas dietary supplementation of Gly-Gln increased VH of the ileum and VH: CD of the duodenum (P < 0.05). The concentration of peripheral blood IL-1 beta was increased by injection of LPS (P < 0.05) and was decreased by dietary Gly-Gln supplementation during the experimental period (P < 0.05); however, there was no interaction of LPS challenge x Gly-Gln addition for IL-1 beta concentration. Concentrations of peripheral blood IL-2 tended to increase at d 14 (P = 0.09) and soluble IL-2 receptor tended to decrease at d 7 (P = 0.06) in piglets supplemented with Gly-Gln; therefore, the peripheral blood IL-2/soluble IL-2 receptor system tended to favor the secretion of IL-2 during the first 2 wk of the experiment. In conclusion, considerable suppression of growth and immune function occurred in early weaning piglets challenged with LPS, and such depression could be alleviated by dietary Gly-Gln supplementation independent of the LPS challenge.