Collection and Characterization of Phloem Exudate from Strawberry Pedicels
Collection and Characterization of Phloem Exudate from Strawberry Pedicels
复制标题
草莓花梗韧皮部分泌物的收集和表征
DOI：
10.21273/hortsci.20.3.413
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发表时间：
1985
期刊：
影响因子：
1.9
通讯作者：
P. Breen
中科院分区：
文献类型：
--
作者：
C. Forney;P. Breen
草莓（Fragaria ×ananassa Duch.）“Brighton”），并将附着于植物的花梗置于乙二胺四乙酸盐（EDTA）中以收集韧皮部汁液。糖渗出率随果实大小的增加而增加，但低于果实在其去除时的干物质积累率。当植物喂14CO_2，放射性出现在韧皮部汁液3小时后，和标记的同化物的渗出率保持在一个相当恒定的速率为6小时。在渗出物中的放射性的92%左右是在蔗糖馏分，其余的葡萄糖（2%），果糖（1%），和酸性和碱性馏分（4%）。结果表明，蔗糖是向果实转运的主要同化物。
Fruit of strawberry (Fragaria ×ananassa Duch. ‘Brighton’) were removed, and pedicels attached to plants were placed in ethylenediaminetetraacetate (EDTA) to collect phloem sap. The rate of sugar exudation increased with the size of the fruit, but was lower than the rate of dry matter accumulation of the fruit at the time of its removal. When plants were fed 14CO2, radioactivity appeared in the phloem sap after 3 hr, and the rate of exudation of labeled assimilate was maintained at a fairly constant rate for 6 hr. About 92% of the radioactivity in the exudate was in the sucrose fraction, with the remaining in glucose (2%), fructose (1%), and acidic and basic fractions (4%). The results indicate that sucrose is the major assimilate translocated to the fruit.