A novel set of single-copy nuclear DNA markers for the genetic study of Salicaceae
A novel set of single-copy nuclear DNA markers for the genetic study of Salicaceae
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DOI：
10.4238/2014.july.4.5
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发表时间：
2014-01-01
影响因子：
0.4
通讯作者：
Zhang, J. G.
中科院分区：
文献类型：
--
作者：
Du, S. H.;Wang, Z. S.;Zhang, J. G.
杨树在世界范围内分布广泛，具有重要的生态和经济价值。然而，缺乏单拷贝核标记限制了对该属的系统发育和群体遗传学的了解。本研究以毛白杨和沙柳的全基因组序列为基础，利用生物信息学方法开发了15个单拷贝核标记的引物对。以山杨和旱柳的20个单株为材料，分别从标记长度和多样性指数两个方面评价了这些标记的基本应用。单拷贝核标记的应用除了提供杨柳科进化动态的信息外，还有望促进对该属的系统发育、种群遗传学和系统地理学的进一步研究。
Species of Populus are widely distributed worldwide, playing a significant role in both ecology and economy. However, the lack of single-copy nuclear markers limits knowledge about the phylogeny and population genetics of this genus. In the present study, primer pairs of 15 single-copy nuclear markers were developed through bioinformatic methods based on complete genomic sequences of Populus trichocarpa and Salix arbutifolia. Twenty individuals of Populus davidiana Dode and Salix matsudana Koidz were used to evaluate the basic application of these markers with respect to marker length and diversity indices, respectively. The utility of single-copy nuclear markers is anticipated to facilitate further studies about the phylogeny, population genetics, and phylogeography of this genus, in addition to providing information about the evolutionary dynamics of Salicaceae.