Construction of Epidermal Growth Factor Receptor Peptide Magnetic Nanovesicles with Lipid Bilayers for Enhanced Capture of Liver Cancer Circulating Tumor Cells
Construction of Epidermal Growth Factor Receptor Peptide Magnetic Nanovesicles with Lipid Bilayers for Enhanced Capture of Liver Cancer Circulating Tumor Cells
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具有脂质双层的表皮生长因子受体肽磁性纳米囊泡的构建用于增强肝癌循环肿瘤细胞的捕获
DOI：
10.1021/acs.analchem.6b01443
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发表时间：
2016-09-20
影响因子：
7.4
通讯作者：
Liang, Xiao-Fei
中科院分区：
文献类型：
--
作者：
Ding, Jian;Wang, Kai;Liang, Xiao-Fei
与抗体和蛋白质相比，多肽是直接靶向低分子量配体的。在本研究中，以肽两亲体（PA）为骨架材料，在不进行表面修饰的情况下，以可控方式设计了含有脂质双分子层的肽磁性纳米载体平台。异硫氰酸荧光素标记的表皮生长因子受体（EGFR）肽纳米颗粒（NPs）可以特异性结合EGFR阳性的肝肿瘤细胞。EGFR肽磁性囊泡（epmv）能有效识别和分离肝癌细胞和处理过的血液样本（磁性epmv与抗epcam NPs的比值：3.5 +/- 0.29）。对32例肝癌患者血液样本的循环肿瘤细胞（CTC）计数分析表明，epmv可有效捕获CTC。因此，这种纳米尺度的靶向货物包装技术可能对设计癌症诊断系统有用。
Highly effective targeted tumor recognition via vectors is crucial for cancer detection In contrast to antibodies and proteins, peptides are direct targeting ligands with a low molecular weight. In the present study, a peptide magnetic nanovector platform containing a lipid bilayer was designed using a peptide amphiphile (PA) as a skeleton material in a controlled manner without surface modification. Fluorescein isothiocyanate-labeled epidermal growth factor receptor (EGFR) peptide nanoparticles (NPs) could specifically bind to EGFR-positive liver tumor cells. EGFR peptide magnetic vesicles (EPMVs) could efficiently recognize and separate hepatoma carcinoma cells from cell solutions and treated blood samples (ratio of magnetic EPMVs versus anti-EpCAM NPs: 3.5 +/- 0.29). Analysis of the circulating tumor cell (CTC) count in blood samples from 32 patients with liver cancer showed that EPMVs Could be effectively applied for CTC capture. Thus, this nanoscale, targeted cargo-packaging technology may be useful for designing cancer diagnostic systems.