DEVELOPMENT OF A CLONAL MYOGENIC CELL LINE WITH UNUSUAL BIOCHEMICAL PROPERTIES
DEVELOPMENT OF A CLONAL MYOGENIC CELL LINE WITH UNUSUAL BIOCHEMICAL PROPERTIES
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DOI：
10.1002/jcp.1040880302
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发表时间：
1976-01-01
影响因子：
5.6
通讯作者：
KLIER, FG
中科院分区：
文献类型：
--
作者：
BRANDT, BL;KIMES, BW;KLIER, FG
研究了亚硝基乙基脲诱导的大鼠肿瘤克隆细胞株B104-F的形态、超微结构、生化和电生理特性与生长阶段的关系。在指数生长的培养物中，细胞是单核的，当电刺激时产生动作电位。固定相培养包含3种类型的细胞：第一类细胞是单核细胞，具有含有微丝和许多平行微管的长突；第二类细胞为单核细胞，不含微管和微丝；第三类细胞具有多核、横纹肌管的超微结构特征。多核细胞产生含有Na和Ca成分的动作电位，并被乙酰胆碱去极化。乙酰胆碱反应被d-管curarine阻断。在肌激酶比活性不变的情况下，固定相培养的肌酸磷酸激酶比活性比指数生长的高9倍。可以利用。细胞的-氨基丁酸含量在固定期比在指数生长培养中高3.5- 26倍，这取决于培养的融合程度。讨论了B104-F的特性与发育中的骨骼肌和中枢神经系统细胞系的特性的关系。
The morphological, ultrastructural, biochemical and electrophysiological properties of B104-F, a clonal cell line derived from a nitrosoethylurea-induced neoplasm in a rat, were studied as a function of the growth phase of the culture. Cells in exponentially growing cultures are mononucleate and produce action potentials when stimulated electrically. Stationary phase cultures contain 3 types of cells: cells of the 1st type are mononucleate and have long processes containing microfilaments and many parallel microtubules; cells of the 2nd type are mononucleate but contain no microtubules and few microfilaments; and cells of the 3rd type have ultrastructural features typical of multinucleate, striated myotubes. Multinucleate cells generate action potentials with both Na and Ca components and are depolarized by acetylcholine. The acetylcholine response is blocked by d-tubocurarine. The specific activity of creatine phosphokinase is 9 times higher in stationary phase cultures than in exponentially growing ones while the myokinase specific activity is unchanged. The .gamma.-aminobutyric acid content of the cells is 3.5- to 26-fold higher in stationary phase than in exponentially growing cultures, depending on the degree of fusion of the culture. The properties of B104-F are discussed in relation to the properties of developing skeletal muscle and of CNS cell lines.