Estimate of true incomplete exchanges using fluorescence in situ hybridization with telomere probes.
Estimate of true incomplete exchanges using fluorescence in situ hybridization with telomere probes.
复制标题
使用端粒探针荧光原位杂交估计真实的不完全交换。
DOI：
10.1080/095530098142068
复制
发表时间：
1998
影响因子：
2.6
通讯作者：
T. Yang
中科院分区：
文献类型：
--
作者：
H. Wu;K. George;T. Yang
PURPOSE
To study the frequency of true incomplete exchanges in radiation-induced chromosome aberrations.
MATERIALS AND METHODS
Human lymphocytes were exposed to 2 Gy and 5 Gy of gamma-rays. Chromosome aberrations were studied using the fluorescence in situ hybridization (FISH) technique with whole chromosome-specific probes, together with human telomere probes. Chromosomes 2 and 4 were chosen in the present study.
RESULTS
The percentage of incomplete exchanges was 27% when telomere signals were not considered. After excluding false incomplete exchanges identified by the telomere signals, the percentage of incomplete exchanges decreased to 11%. Since telomere signals appear on about 82% of the telomeres, the percentage of true incomplete exchanges should be even lower and was estimated to be 3%. This percentage was similar for chromosomes 2 and 4 and for doses of both 2 Gy and 5 Gy.
CONCLUSIONS
The percentage of true incomplete exchanges is significantly lower in gamma-irradiated human lymphocytes than the frequencies reported in the literature.
影响因子：
3.4
作者：
M. Cornforth;J. Meyne;L. Littlefield;S. Bailey;R. Moyzis
通讯作者：
M. Cornforth;J. Meyne;L. Littlefield;S. Bailey;R. Moyzis
分析人类 1、2 和 4 号染色体中自然发生的和辐射引起的断点位置。
影响因子：
3.4
作者：
Tucker,JD;Senft,JR
通讯作者：
Senft,JR
辐射产生的染色体畸变：过多的无着丝粒片段与双着丝粒之间的关系。
DOI：
--
发表时间：
1995
期刊：
Radiation research.
影响因子：
--
作者：
Hahnfeldt,P;Hlatky,LR;Brenner,DJ;Sachs,RK
通讯作者：
Sachs,RK