Adenosine plasma level correlates with homocysteine and uric acid concentrations in patients with coronary artery disease
Adenosine plasma level correlates with homocysteine and uric acid concentrations in patients with coronary artery disease
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DOI：
10.1139/cjpp-2015-0193
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发表时间：
2016-03-01
影响因子：
2.1
通讯作者：
Guieu, R.
中科院分区：
文献类型：
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作者：
Fromonot, J.;Deharo, P.;Guieu, R.
高同型半胱氨酸血症在冠心病（CAD）患者中的作用尚不清楚。本研究使用SYNTAX评分评估同型半胱氨酸（HCys）、腺苷血浆浓度（APC）、血浆尿酸和冠心病严重程度之间的关系。我们还在体外评估了腺苷对肝癌培养细胞（HuH7）产生HCys的影响。纳入78例患者（平均年龄+/- SD: 66.3 +/- 11.3；平均SYNTAX评分：19.9 +/- 12.3）和30例健康受试者（平均年龄：61 +/- 13）。我们增加腺苷浓度培养HuH7细胞，并在细胞培养上清中研究对HCys水平的影响。与对照组相比，患者APC （0.82 +/- 0.5 mu mol/L vs 0.53 +/- 0.14 mu mol/L, p < 0.01）、HCys （15 +/- 7.6 mu mol/L vs 6.8 +/- 3 mu mol/L, p < 0.0001）和尿酸（242.6 +/- 97 vs 202 +/- 59, p < 0.05）水平较高。APC与患者HCys和尿酸浓度相关（Pearson’s R分别为0.65和0.52,p < 0.0001）。SYNTAX评分与HCys浓度相关。腺苷诱导细胞培养中HCys呈时间和剂量依赖性增加。我们的数据表明，高APC与冠心病患者的HCys和尿酸浓度有关。APC升高是否参与动脉粥样硬化，或者相反，是保护调节过程的一部分，需要进一步研究。
The role of hyperhomocysteinemia in coronary artery disease (CAD) patients remains unclear. The present study evaluated the relationship between homocysteine (HCys), adenosine plasma concentration (APC), plasma uric acid, and CAD severity evaluated using the SYNTAX score. We also evaluated in vitro the influence of adenosine on HCys production by hepatoma cultured cells (HuH7). Seventy-eight patients (mean age +/- SD: 66.3 +/- 11.3; mean SYNTAX score: 19.9 +/- 12.3) and 30 healthy subjects (mean age: 61 +/- 13) were included. We incubated HuH7 cells with increasing concentrations of adenosine and addressed the effect on HCys level in cell culture supernatant. Patients vs. controls had higher APC (0.82 +/- 0.5 mu mol/L vs 0.53 +/- 0.14 mu mol/L; p < 0.01), HCys (15 +/- 7.6 mu mol/L vs 6.8 +/- 3 mu mol/L, p < 0.0001), and uric acid (242.6 +/- 97 vs 202 +/- 59, p < 0.05) levels. APC was correlated with HCys and uric acid concentrations in patients (Pearson's R = 0.65 and 0.52; p < 0.0001, respectively). The SYNTAX score was correlated with HCys concentration. Adenosine induced a time- and dose-dependent increase in HCys in cell culture. Our data suggest that high APC is associated with HCys and uric acid concentrations in CAD patients. Whether the increased APC participates in atherosclerosis or, conversely, is part of a protective regulation process needs further investigations.