Effect of deglycosylation of cellobiose dehydrogenases on the enhancement of direct electron transfer with electrodes.
Effect of deglycosylation of cellobiose dehydrogenases on the enhancement of direct electron transfer with electrodes.
复制标题
纤维二糖脱氢酶的去糖基化对增强电极直接电子转移的影响。
DOI：
10.1021/ac3022899
复制
发表时间：
2012
影响因子：
7.4
通讯作者：
L. Gorton
中科院分区：
文献类型：
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作者：
R. Ortiz;H. Matsumura;F. Tasca;Kawah Zahma;M. Samejima;K. Igarashi;R. Ludwig;L. Gorton
纤维二糖脱氢酶（CDH）是单体细胞外黄素细胞色素，其由含有黄素腺嘌呤二核苷酸（FAD）的催化脱氢酶结构域（DH（CDH））、含有血红素B的细胞色素结构域（CYT（CDH））和连接两个结构域的接头区组成。本文研究了去糖基化对黄孢原毛平革菌（PcCDH）和拟蜡孔菌（CsCDH）CDH电化学性质的影响。所有糖基化和去糖基化的酶都显示出CYT（CDH）和电极之间的直接电子转移（DET）。用去糖基化的PcCDH（dPcCDH）和CsCDH（dCsCDH）修饰的石墨电极在底物存在下具有比用它们的糖基化对应物修饰的电极高40-65%的I（max）值。CsCDH被困在选择性渗透膜下的巯基自组装单分子层（SAM）保护的金电极上显示出类似的变化，相反，PcCDH的去糖基化没有表现出任何不同的电催化响应SAM修饰的金电极。使用动态光散射，发现糖基化PcCDH具有比dPcCDH大30%的流体动力学半径。介绍了生物电化学的基本原理和生物电催化特性。
Cellobiose dehydrogenase (CDH) is a monomeric extracellular flavocytochrome composed of a catalytic dehydrogenase domain (DH(CDH)) containing flavin adenine dinucleotide (FAD), a cytochrome domain (CYT(CDH)) containing heme b, and a linker region connecting the two domains. In this work, the effect of deglycosylation on the electrochemical properties of CDH from Phanerochaete chrysosporium (PcCDH) and Ceriporiopsis subvermispora (CsCDH) is presented. All the glycosylated and deglycosylated enzymes show direct electron transfer (DET) between the CYT(CDH) and the electrode. Graphite electrodes modified with deglycosylated PcCDH (dPcCDH) and CsCDH (dCsCDH) have a 40-65% higher I(max) value in the presence of substrate than electrodes modified with their glycosylated counterparts. CsCDH trapped under a permselective membrane showed similar changes on gold electrodes protected by a thiol-based self-assembled monolayer (SAM), in contrast to PcCDH for which deglycosylation did not exhibit any different electrocatalytical response on SAM-modified gold electrodes. Glycosylated PcCDH was found to have a 30% bigger hydrodynamic radius than dPcCDH using dynamic light scattering. The basic bioelectrochemistry as well as the bioelectrocatalytic properties are presented.
M. Yoshida、T. Ohira、K. Igarashi、H. Nagasawa、K. Aida、B. M. Hallberg、C. Divne、T. Nishino 和 M. Samejima：“甲基营养酵母中重组原毛平革菌纤维二糖脱氢酶的生产和表征
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