Isolation and purification of primary rodent astrocytes.
Isolation and purification of primary rodent astrocytes.
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DOI：
10.1002/0471142301.ns0305s00
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Weinstein, D E
中科院分区：
文献类型：
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作者：
Weinstein, D E
星形胶质细胞是哺乳动物中枢神经系统（CNS）的主要细胞类型。获得这些细胞的几乎纯群体的能力使得有可能研究它们作为分离细胞或混合群体的功能，在混合群体中它们支持周围神经元的生长和存活。与其他成熟的中枢神经系统细胞不同，成熟的星形胶质细胞保持终身重新进入细胞周期的能力。本单元中描述的第一种分离方法利用了这些细胞的增殖能力，第二种方法也是如此，只是不需要抗体或补体处理。胶质细胞酸性蛋白（GFAP），这是存在于大多数星形胶质细胞在体内和几乎所有的星形胶质细胞在体外，是一个有用的标志物，用于评估培养物的纯度检测程序，也提出。
Astrocytes are a major cell type in the mammalian central nervous system (CNS). The ability to obtain virtually pure populations of these cells makes it possible to study their function as isolated cells or in mixed populations where they support the growth and survival of surrounding neurons. Unlike other mature CNS cells, mature astrocytes maintain the lifelong ability to reenter the cell cycle. The first isolation procedure described in this unit takes advantage of the proliferative ability of these cells, as does the second, except that no antibody or complement treatment is required. A procedure for detecting glial fibrillary acidic protein (GFAP), which is present in most astrocytes in vivo and virtually all astrocytes in vitro and is a useful marker for assessing the purity of cultures, is also presented.