New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine.
New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine.
复制标题
利用三氯均三嗪制备生物特异性吸附剂和固定化酶的新方法。
DOI：
10.1016/0003-2697(78)90571-7
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发表时间：
1978
影响因子：
2.9
通讯作者：
L. T. Hodgins
中科院分区：
文献类型：
--
作者：
T. Finlay;V. Troll;M. Levy;A. Johnson;L. T. Hodgins
描述了利用 Sepharose CL 作为载体、三氯均三嗪作为连接剂制备生物特异性吸附剂和固定化酶的方法。通过在无水有机相中进行活化反应并用芳香胺取代三嗪环上的第二个氯，避免了三氯-s-三嗪与不溶性多元醇的常规水相和混合水相反应，特别是试剂水解过程中遇到的困难。配体可以在水相或有机相中与活化的载体偶联。该方法已应用于将许多不同的酶、蛋白质和小分子配体附着到琼脂糖凝胶上。证明了三嗪键相对于溴化氰键的优越性。
Methods for preparing biospecific adsorbents and immobilized enzymes utilizing Sepharose CL as a support and trichloro-s-triazine as the linking agent are described. The difficulties encountered during conventional aqueous and mixed aqueous-phase reactions of trichloro-s-triazine with insoluble polyols, particularly reagent hydrolysis, are avoided by performing the activation reactions in anhydrous organic phase and replacing the second chlorine on the triazine ring by an aromatic amine. Ligands can be coupled to the activated support in either aqueous or organic phase. The methods have been applied to the attachment of a number of different enzymes, proteins, and small-molecule ligands to Sepharose. The superiority of the triazine linkage to the cyanogen bromide linkage is demonstrated.