Superabsorbent 3D Scaffold Based on Electrospun Nanofibers for Cartilage Tissue Engineering
Superabsorbent 3D Scaffold Based on Electrospun Nanofibers for Cartilage Tissue Engineering
复制标题
用于软骨组织工程的基于静电纺丝纳米纤维的超吸水性 3D 支架
DOI：
10.1021/acsami.6b06825
复制
发表时间：
2016-09-21
影响因子：
9.5
通讯作者：
Mo, Xiumei
中科院分区：
文献类型：
--
作者：
Chen, Weiming;Chen, Shuai;Mo, Xiumei
静电纺丝纳米纤维已用于各种生物医学应用。然而，静电纺丝通常产生二维 (2D) 膜，这限制了纳米纤维在 3D 组织工程支架中的应用。在本研究中，多孔 3D 支架。 (3DS-1) 基于电纺明胶/PLA 纳米纤维，已制备用于软骨组织再生。为了进一步提高软骨的修复效果，还成功制备了与透明质酸（HA）交联的改良支架（3DS-2）。研究了3D支架的纳米纤维结构、吸水率和压缩力学性能。在 3D 支架上培养软骨细胞，并检查其活力和形态。还使用关节软骨损伤模型对 3D 支架进行了兔子体内软骨再生研究。结果表明，3DS-1和3DS-2表现出超吸收性能和优异的细胞相容性。这两种支架在潮湿状态下均呈现弹性：体内研究表明3DS-2可以增强软骨的修复。目前的 3D 纳米纤维支架 (3DS-2) 在软骨组织工程应用中具有广阔的前景。
Electrospun nanofibers have been used for various biomedical applications. However, electrospinning commonly produces two-dimensional (2D) membranes, which limits the application of nanofibers for the 3D tissue engineering scaffold. In the present study, a porous 3D scaffold. (3DS-1) based on electrospun gelatin/PLA nanofibers has been prepared for cartilage tissue regeneration. To further improve the repairing:effect Of cartilage, a modified scaffold (3DS-2) cross-linked with hyaluronic acid (HA) was also successfully fabricated. The nanofibrous structure, water absorption, and compressive mechanical properties of 3D scaffold were studied. Chondrocytes were cultured on 3D scaffold, and their viability and morphology were examined. 3D scaffolds were also subjected to,an in vivo cartilage regeneration study on rabbits using an articular cartilage injury model. The results indicated that 3DS-1 and 3DS-2 exhibited superabsorbent property and excellent cytocompatibility. Both these scaffolds present elastic property in the wet state: An in vivo study showed that 3DS-2 could enhance the repair of cartilage. The present 3D nanofibrous scaffold (3DS-2) would be promising for cartilage tissue engineering application.