Isolation, characterization, and organ-specific expression of two novel human zinc finger genes related to the Drosophila gene spalt
Isolation, characterization, and organ-specific expression of two novel human zinc finger genes related to the Drosophila gene spalt
复制标题
DOI：
10.1006/geno.1996.0631
复制
发表时间：
1996-12-15
期刊：
影响因子：
4.4
通讯作者：
Stick, R
中科院分区：
文献类型：
--
作者：
Kohlhase, J;Schuh, R;Stick, R
果蝇的区域特异性同源异型基因spalt（sal）指定头部和尾部，而不是躯干部分。在个体发育的后期阶段，sal也在发育胚胎的少数组织和器官中表达和需要。sal编码一种结构独特的锌指蛋白。我们利用这些独特的功能，从人类中分离的sal-like基因，在这里，我们报告的分离和分子特征的两个sal-like转录单位，命名为Hsal 1和Hsal 2，分别位于染色体16q12.1和14q11.1-q12.1。它们的转录本在有限数量的成人器官中表达，包括大脑。虽然Hsal 2在不同的脑区均匀表达，但Hsal 1转录物优先积累在胼胝体和黑质中。在胎儿脑中，这两个基因的转录本中检测到的神经元中，sal-like锌指结构域的排列和它们的高度序列相似性表明，一个新的和保守的人类锌指转录因子的亚家族，是密切相关的果蝇基因产物由基因sal编码。(C)出版社：Academic Press，Inc.
The region-specific homeotic gene spalt (sal) of Drosophila specifies head and tail as opposed to trunk segments. During later stages of ontogenesis, sal is also expressed and required in a small number of tissues and organs in the developing embryo. sal encodes a zinc finger protein of unusual but characteristic structure. We made use of these unique features to isolate sal-like genes from humans, Here we report the isolation and molecular characterization of two sal-like transcription units, termed Hsal1 and Hsal2, which are located on chromosomes 16q12.1 and 14q11.1-q12.1, respectively. Their transcripts are expressed in a limited number of adult organs, including the brain. While Hsal2 is evenly expressed in different brain areas, Hsal1 transcripts preferentially accumulate in the corpus callosum and the substantia nigra. In the fetal brain, transcripts of both genes were detected in neurons, The arrangement of sal-like zinc finger domains and their high degree of sequence similarity suggest a novel and conserved subfamily of human zinc finger transcription factors that is closely related to the Drosophila gene product encoded by the gene sal. (C) 1996 Academic Press, Inc.