Recent trends in protein biochip technology.
Recent trends in protein biochip technology.
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DOI：
10.1517/14622416.1.4.395
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发表时间：
2000-11
期刊：
影响因子：
2.1
通讯作者：
S. Weinberger;Tina S. Morris;Michael Pawlak
中科院分区：
文献类型：
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作者：
S. Weinberger;Tina S. Morris;Michael Pawlak
本文介绍了目前的趋势和进展，蛋白质生物芯片技术，依赖于从液体介质中提取和保留的目标蛋白质。这些不断发展的平台的分析优势以及技术挑战，特别强调在后基因组时代的选择性，灵敏度，吞吐量和实用性。本文综述了蛋白质生物芯片技术的研究进展，介绍了蛋白质生物芯片的结构、操作方法以及蛋白质检测技术。重点讨论了三种蛋白质生物芯片技术，生物分子相互作用分析（Biacore，乌普萨拉，瑞典），表面增强激光解吸/电离（SELDI）蛋白质芯片阵列（Ciphergen Biosystems，弗里蒙特，CA，美国）和荧光平面波导（Zeptosens，Witterswil，瑞士），沿着相关应用的例子。
This article presents current trends and advances in protein biochip technologies that rely upon extraction and retention of target proteins from liquid media. Analytical strengths as well as technical challenges for these evolving platforms are presented with particular emphasis on selectivity, sensitivity, throughput and utility in the post-genome era. A general review of protein biochip technology is provided, which delineates approaches for protein biochip format and operation, as well as protein detection. A focused discussion of three protein biochip technologies, Biomolecular Interaction Analysis (Biacore, Uppsala, Sweden), Surface Enhanced Laser Desorption/Ionisation (SELDI) ProteinChip Arrays (Ciphergen Biosystems, Fremont, CA, USA) and Fluorescent Planar Wave Guide (Zeptosens, Witterswil, Switzerland), follows along with examples of relevant applications.