Quantitative long-term measurements of burns in a rat model using Spatial Frequency Domain Imaging (SFDI) and Laser Speckle Imaging (LSI).
Quantitative long-term measurements of burns in a rat model using Spatial Frequency Domain Imaging (SFDI) and Laser Speckle Imaging (LSI).
复制标题
DOI：
10.1002/lsm.22647
复制
发表时间：
2017-03
影响因子：
2.4
通讯作者：
Durkin AJ
中科院分区：
文献类型：
--
作者：
Ponticorvo A;Burmeister DM;Rowland R;Baldado M;Kennedy GT;Saager R;Bernal N;Choi B;Durkin AJ
目前诊断烧伤严重程度和随后伤口愈合的标准是通过临床检查，这是高度主观的。一些新技术正在将重点转移到烧伤护理上，不仅试图量化烧伤深度，还试图量化愈合的进展。虽然对部分厚度烧伤的准确早期评估对于决定治疗过程至关重要，但随着时间的推移定量监测伤口状态的能力对于了解治疗效果至关重要。SFDI和LSI都是非侵入性成像方式，已被证明对烧伤严重程度有很大的诊断价值，但尚未在伤口愈合过程中进行测试。在这项研究中，无毛大鼠模型（n= 6,300 -450g）与四齿梳一起创建四个相同的部分厚度烧伤（浅n=3和深n=3），用于监测伤口愈合，为期28天。每周进行活检进行组织学分析，以验证伤口进展。每周进行SFDI和LSI，以跟踪烧伤血流动力学（血流量和血氧饱和度）和结构（降低散射系数）特性的演变。到第7天，LSI显示血流量从基线变化到浅表烧伤的220%和深度烧伤的165%。在浅表烧伤中，血流量在第28天恢复到基线水平，但在深度烧伤中，血流量仍然升高。在同一时期，周围组织的血流量也有较小的增加。用SFDI测量的氧饱和度值显示，到第28天，浅表烧伤的氧饱和度值从基线值66%逐渐增加到74%，深度烧伤的氧饱和度值为72%。此外，SFDI在烧伤产生后不久就显示出降低的散射系数显著降低。散射系数在伤口区域逐渐下降，但在28天结束时恢复到基线水平。尽管存在血流动力学变化，但周围组织的散射变化保持不变。本研究表明，LSI和SFDI能够监测烧伤创面28天内血流动力学和散射特性的变化。这些结果强调了使用非侵入性成像技术研究伤口愈合可以获得的潜在见解。这些技术的进一步发展对于伤口监测和研究不同治疗方法的疗效可能是革命性的。
The current standard for diagnosis of burn severity and subsequent wound healing is through clinical examination, which is highly subjective. Several new technologies are shifting focus to burn care in an attempt to help quantify not only burn depth but also the progress of healing. While accurate early assessment of partial thickness burns is critical for dictating the course of treatment, the ability to quantitatively monitor wound status over time is critical for understanding treatment efficacy. SFDI and LSI are both non-invasive imaging modalities that have been shown to have great diagnostic value for burn severity, but have yet to be tested over the course of wound healing. In this study, a hairless rat model (n=6, 300-450g) was used with a four pronged comb to create four identical partial thickness burns (superficial n=3 and deep n=3) that were used to monitor wound healing over a 28 day period. Weekly biopsies were taken for histological analysis to verify wound progression. Both SFDI and LSI were performed weekly to track the evolution of hemodynamic (blood flow and oxygen saturation) and structural (reduced scattering coefficient) properties for the burns. LSI showed significant changes in blood flow from baseline to 220% in superficial and 165% in deep burns by day 7. In superficial burns, blood flow returned to baseline levels by day 28, but not for deep burns where blood flow remained elevated. Smaller increases in blood flow were also observed in the surrounding tissue over the same time period. Oxygen saturation values measured with SFDI showed a progressive increase from baseline values of 66% to 74% in superficial burns and 72% in deep burns by day 28. Additionally, SFDI showed significant decreases in the reduced scattering coefficient shortly after the burns were created. The scattering coefficient progressively decreased in the wound area, but returned towards baseline conditions at the end of the 28 day period. Scattering changes in the surrounding tissue remained constant despite the presence of hemodynamic changes. Here we show that LSI and SFDI are capable of monitoring changes in hemodynamic and scattering properties in burn wounds over a 28 day period. These results highlight the potential insights that can be gained by using noninvasive imaging technologies to study wound healing. Further development of these technologies could be revolutionary for wound monitoring and studying the efficacy of different treatments.