IMMUNOLOGICAL DETECTION OF ACTIN IN THE 14S CILIARY DYNEIN OF TETRAHYMENA
IMMUNOLOGICAL DETECTION OF ACTIN IN THE 14S CILIARY DYNEIN OF TETRAHYMENA
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DOI：
10.1016/0014-5793(94)80313-7
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发表时间：
1994-04-25
期刊：
影响因子：
3.5
通讯作者：
KAMIYA, R
中科院分区：
文献类型：
--
作者：
MUTO, E;EDAMATSU, M;KAMIYA, R
肌动蛋白与四膜虫纤毛动力蛋白的协会进行了检查，使用针对四膜虫肌动蛋白的多克隆抗体。蛋白质印迹显示，肌动蛋白存在于148动力蛋白组分中，但不存在于22 S动力蛋白组分中，22 S动力蛋白组分包括外臂。通过阴离子交换色谱，14 S动力蛋白可以进一步分离成三个主要组分，总共含有四个不同的重链。当通过抗肌动蛋白免疫印迹测试每个级分时，所有三个级分都含有与重链接近化学计量量的肌动蛋白。由于四膜虫肌动蛋白与其他物种的肌动蛋白显著不同，与内臂动力蛋白的关联可能是肌动蛋白的保守性质。
The association of actin with Tetrahymena ciliary dyneins was examined using a polyclonal antibody against Tetrahymena actin. Western blotting shows that actin is present in the 148 dynein fraction, but not in the 22S dynein fraction, which comprises the outer arm. By anion-exchange chromatography, 14S dynein can be further separated into three major fractions that contain four distinct heavy chains in total. When each fraction was tested by anti-actin immunoblotting, all three fractions contained actin in nearly stoichiometric amounts with the heavy chain. Since Tetrahymena actin differs significantly from actins of other species, the association with inner-arm dynein may be a conserved property of actin.