Comparative growth behaviour and characterization of stem cells from human Wharton's jelly
Comparative growth behaviour and characterization of stem cells from human Wharton's jelly
复制标题
DOI：
10.1016/s1472-6483(10)60539-1
复制
发表时间：
2007-12-01
影响因子：
4
通讯作者：
Bongso, A.
中科院分区：
文献类型：
--
作者：
Fong, C. Y.;Richards, M.;Bongso, A.
人类胚胎干细胞（hESQ）面临着伦理敏感性和畸胎瘤形成的问题。虽然沃顿胶质干细胞（WJSC），也是胚胎来源，可能不会面临这样的伦理问题，它是不确定的hESC培养条件下，他们是否会作为hESC的多能性。在塑料上生长的WJSC在原代培养中显示出两种类型的形态（上皮样和短成纤维细胞），这取决于所使用的培养基，并且在传代时仅显示成纤维细胞形态。当在hESC培养基存在下在小鼠饲养细胞上生长时，它们产生含有具有高核质比和突出核仁的hESC样细胞的非典型集落。它们对hESC标志物Tra-1-60、Tra-1-81、SSEA-1、SSEA-4、Oct-4和碱性磷酸酶呈阳性，对SSEA-3呈阴性，显示正常核型，发育胚状体（EB）样结构，在SCID小鼠中不产生畸胎瘤，并分化成神经元衍生物。它们也对间充质CD标记物（CD 105，CD 90，CD 44）呈阳性，对CD 34和HLA呈阴性，尽管10个胚胎干细胞基因组标记物中有9个是可检测的，但这些标记物的表达水平较低。因此，WJSC不像hESC那样是多能的，但具有广泛的多能性，并且具有能够容易地按比例放大并且不诱导畸胎瘤的优点。
Human embryonic stem cells (hESQ,face ethical sensitivities and the problem of teratoma formation. Although Wharton's jelly stem cells (WJSC), also of embryonic origin, may not face such ethical concerns, it is not definitely known whether under hESC culture conditions they would be as pluripotent as hESC. WJSC grown on plastic showed two types of morphology (epithelioid and short fibroblastic) in primary culture depending on the culture medium used, and only fibroblastic morphology when passaged. When grown in the presence of hESC medium on mouse feeder cells, they produced atypical colonies containing hESC-like cells with high-nuclear cytoplasmic ratios and prominent nucleoli. They were positive for the hESC markers Tra-1-60, Tra-1-81, SSEA-1, SSEA-4 Oct-4 and alkaline phosphatase, negative for SSEA-3, showed normal karyotypes, developed embryoid body (EB)-like structures, did not produce teratomas in SCID mice and differentiated into neuronal derivatives. They were also positive for the mesenchymal CD markers (CD105, CD90, CD44), negative for CD34 and HLA, and although nine out of 10 embryonic stem cell genomic markers were detectable, these were expressed at low levels. WJSC are thus not as pluripotent as hESC but widely multipotent, and have the advantages of being able to be scaled up easily and not inducing teratomas.