Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress
Ca2+ involved in GABA signal transduction for phenolics accumulation in germinated hulless barley under NaCl stress
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Ca2 参与 NaCl 胁迫下发芽大麦酚类物质积累的 GABA 信号转导
DOI：
10.1016/j.fochx.2019.100023
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发表时间：
2019-06-30
期刊：
影响因子：
6.1
通讯作者：
Yang, Runqiang
中科院分区：
文献类型：
--
作者：
Ma, Yan;Wang, Pei;Yang, Runqiang
本研究以大麦幼苗为材料，用外源GABA及其合成抑制剂3-巯基丙酸(3-MP)、钙通道阻断剂La3+、钙离子螯合剂EGTA和钙释放通道抑制剂2-氨基乙氧基二苯硼酸酯(2-APB)处理大麦幼苗，以探讨钙离子在大麦幼苗酚类物质积累中的作用。结果表明，GABA显著提高了酚类、钙和钙调蛋白的含量。诱导大麦根尖细胞内钙离子内流，改变钙离子的分布，使钙沉淀更均匀、更密集。而3-MP处理则表现出相反的变化，说明GABA对钙含量的增加是必需的。此外，LaCl3、EGTA和2-APB处理抑制了酚类物质的积累，外源GABA可以部分缓解这种抑制作用。综上所述，Ca~(2+)参与了盐胁迫下大麦幼苗酚类物质积累的GABA信号转导过程。
In this study, in order to investigate the role of Ca2+ in GABA signal transduction involved in phenolics accumulation in barley seedlings under NaCl stress, the seedlings were treated with exogenous GABA and its synthesis inhibitor, 3-mercaplopropionic acid (3-MP), as well as Ca2+ channel blockers La3+, Ca2+ chelator EGTA, and Ca2+ release channel inhibitor 2-aminoethoxydiphenyl borate (2-APB). The results showed that GABA significantly enhanced phenolics, calcium and calmodulin content. It also induced Ca2+ influx in barley root tips cells, and altered the distribution of Ca2+, making calcium precipitates more uniform and intensive. While, 3-MP treatment led to opposite changes, which suggested that GABA was essential for calcium content increase. In addition, accumulation of phenolics was inhibited by LaCl3, EGTA and 2-APB treatments, and this inhibition could be alleviated partly by exogenous GABA. Taken together, Ca2+ was involved in GABA signal transduction for phenolics accumulation in barley seedlings under NaCl stress.