Conservation of mRNA and Protein Expression during Development of C.elegans
Conservation of mRNA and Protein Expression during Development of C.elegans
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DOI：
10.1016/j.celrep.2014.01.001
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发表时间：
2014-02-01
期刊：
影响因子：
8.8
通讯作者：
Rajewsky, Nikolaus
中科院分区：
文献类型：
--
作者：
Gruen, Dominic;Kirchner, Marieluise;Rajewsky, Nikolaus
基因表达的时空控制对发育至关重要，并受到进化变化的影响。虽然蛋白质是大多数基因的最终产物，但动物的发育蛋白质组尚未得到全面定义，并且mRNA与发育过程中蛋白质丰度之间的相关性在很大程度上是未知的。在这里，我们在全球范围内测量并比较了秀丽隐杆线虫（C. elegans）和秀丽隐杆线虫（C. briggsae）生命周期中蛋白质和mRNA的表达变化，这两种线虫的进化间隔约为3000万年。我们观察到发育mRNA和蛋白质的变化高度保守到惊人的相似程度，但在一个物种内相关性很差，这表明重要且广泛的转录后调控。如果mRNAfold的变化在蛋白水平上被缓冲，则转录后控制尤其保守，表明其具有主要的抑制功能。最后，在不同表达的基因中，我们确定了胰岛素信号通路，这是一种参与寿命决定的途径，是适应性进化的假定目标。
Spatiotemporal control of gene expression is crucial for development and subject to evolutionary changes. Although proteins are the final product of most genes, the developmental proteome of an animal has not yet been comprehensively defined, and the correlation between mRNA and protein abundance during development is largely unknown. Here, we globally measured and compared protein and mRNA expression changes during the life cycle of the nematodes C. elegans and C. briggsae, separated by similar to 30 million years of evolution. We observed that developmental mRNA and protein changes were highly conserved to a surprisingly similar degree but were poorly correlated within a species, suggesting important and widespread posttranscriptional regulation. Posttranscriptional control was particularly well conserved if mRNAfold changeswere bufferedon the protein level, indicating a predominant repressive function. Finally, among divergently expressed genes, we identified insulin signaling, a pathway involved in lifespan determination, as a putative target of adaptive evolution.