Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation.
Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation.
复制标题
酿酒酵母核孔蛋白 Nup170p 在染色体分离中的新作用。
DOI：
10.1093/genetics/157.4.1543
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发表时间：
2001
期刊：
影响因子：
3.3
通讯作者：
Basrai,MA
中科院分区：
文献类型：
--
作者：
Kerscher,O;Hieter,P;Winey,M;Basrai,MA
我们确定核孔蛋白基因NUP170的突变导致酿酒酵母染色体传递保真度（ctf）和动粒完整性的缺陷。Actf突变株，termeds141，表现出转录通读表型，并在两个着丝粒完整性试验中稳定了双着丝粒染色体片段。以前，这些测定导致两个基本的动粒组分，Ctf13 p和Ctf14 p的鉴定。因此，s141代表另一个参与维持动粒完整性的突变体。我们克隆并定位了s141突变的互补基因，结果表明它与S.酿酒酵母NUP170基因。NUP170的缺失株（nup170 Δ：：HIS3）具有与141突变体（nup170 - 141）相似的Ctf-表型，并且也表现出动粒完整性缺陷。我们鉴定了第二个核孔蛋白NUP157，NUP170的同源物，作为nup170 - 141和nup170 Δ：：HIS 3菌株的Ctf-表型的抑制因子。然而，NUP157或其他几个核孔蛋白的缺失并不影响染色体分离。我们的数据表明NUP170编码一种特殊的核孔蛋白，在染色体分离和可能的动粒功能中具有独特的作用。
We determined that a mutation in the nucleoporin geneNUP170leads to defects in chromosome transmission fidelity (ctf) and kinetochore integrity inSaccharomyces cerevisiae. Actfmutant strain, termeds141, shows a transcription readthrough phenotype and stabilizes a dicentric chromosome fragment in two assays for kinetochore integrity. Previously, these assays led to the identification of two essential kinetochore components, Ctf13p and Ctf14p. Thus,s141represents anotherctfmutant involved in the maintenance of kinetochore integrity. We cloned and mapped the gene complementing thectfmutation ofs141and showed that it is identical to theS. cerevisiae NUP170gene. A deletion strain ofNUP170(nup170Δ::HIS3) has aCtf–phenotype similar to thes141mutant (nup170-141) and also exhibits a kinetochore integrity defect. We identified a second nucleoporin,NUP157, a homologue ofNUP170, as a suppressor of theCtf–phenotype ofnup170-141andnup170Δ::HIS3strains. However, a deletion ofNUP157or several other nucleoporins did not affect chromosome segregation. Our data suggest thatNUP170encodes a specialized nucleoporin with a unique role in chromosome segregation and possibly kinetochore function.