Surgery Trauma Severity but not Anesthesia Length Contributes to Postoperative Cognitive Dysfunction in Mice
Surgery Trauma Severity but not Anesthesia Length Contributes to Postoperative Cognitive Dysfunction in Mice
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手术创伤严重程度而非麻醉时间会导致小鼠术后认知功能障碍
DOI：
10.3233/jad-201232
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发表时间：
2021-01-01
影响因子：
4
通讯作者：
Zuo, Zhiyi
中科院分区：
文献类型：
--
作者：
Lai, Zhongmeng;Min, Jia;Zuo, Zhiyi
背景资料：围手术期，可修改的因素，有助于围手术期神经认知障碍（PND）尚未明确defined.Objective：以确定的贡献麻醉长度和程度的手术创伤，PND和神经炎症，一个关键的过程，PND。将三个月大的C57 BL/6 J小鼠进行2小时或6小时的异氟烷麻醉加上5分钟或15分钟的左颈总动脉暴露（手术）。在析因设计中（两个因素：两个节段的麻醉和三个节段的手术）。用巴恩斯迷宫和新物体识别实验测试大鼠的学习记忆能力。收集血液、脾脏和海马用于测量白细胞介素（IL）-6和IL-1 β。18月龄C57 BL/6 J小鼠（老年小鼠）分别接受异氟醚麻醉6 h或异氟醚麻醉2 h+手术15 min，然后进行学习记忆测试。单独麻醉或麻醉加5分钟手术不影响小鼠在这些试验中的表现。同样，只有长时间手术的小鼠血液、脾脏和海马中的IL-6和IL-1 β增加，脾脏重量降低，而其他实验条件下的小鼠没有。术后在海马中发现脾细胞。同样，老年小鼠手术时间长，但不是单独使用异氟烷麻醉的小鼠在巴恩斯迷宫和新物体识别tests.Conclusion：手术创伤，但不是麻醉，有助于PND和神经炎症的发展。脾细胞可以调节这些过程。
Background: Perioperative, modifiable factors contributing to perioperative neurocognitive disorders (PND) have not been clearly defined.Objective: To determine the contribution of anesthesia lengths and the degrees of surgical trauma to PND and neuroinflammation, a critical process for PND.Methods: Three-month-old C57BL/6J mice were subjected to 2 h or 6 h isoflurane anesthesia plus a 5 min or 15 min left common carotid artery exposure (surgery) in a factorial design (two factors: anesthesia with two levels and surgery with three levels). Their learning and memory were tested by Barnes maze and novel object recognition paradigms. Blood, spleen, and hippocampus were harvested for measuring interleukin (IL)-6 and IL-1 beta. Eighteen-month-old C57BL/6J mice (old mice) were subjected to 6 h isoflurane anesthesia or 2 h isoflurane anesthesia plus 15 min surgery and then had learning and memory tested.Results: Three-month-old mice with 15 min surgery (long surgery) under 2 h or 6 h anesthesia performed poorly in the learning and memory tests compared with controls. Anesthesia alone or anesthesia plus 5 min surgery did not affect mouse performance in these tests. Similarly, only mice with long surgery but not mice with other experimental conditions had increased IL-6 and IL-1 beta in the blood, spleen, and hippocampus and decreased spleen weights. Splenocytes were found in the hippocampus after surgery. Similarly, old mice with long surgery but not the mice with isoflurane anesthesia alone had poor performance in the Barnes maze and novel object recognition tests.Conclusion: Surgical trauma, but not anesthesia, contributes to the development of PND and neuroinflammation. Splenocytes may modulate these processes.