Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration.
Circulating monocytes and B-lymphocytes in neovascular age-related macular degeneration.
复制标题
新生血管相关的黄斑变性中的循环单核细胞和B淋巴细胞。
DOI：
10.2147/opth.s121332
复制
发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Sørensen TL
中科院分区：
文献类型：
--
作者：
Hector SM;Sørensen TL
患有新生血管性年龄相关性黄斑变性（AMD）的个体具有改变的视网膜巨噬细胞的数量和分布，并且显示循环抗体的变化。我们想调查相应的前体，亚群。因此，我们测量了单核细胞和B淋巴细胞群体与新生血管性AMD的个人。这是一项观察性病例对照研究。共纳入31名新生血管性AMD患者和30名年龄匹配的健康对照者。对患者和对照组进行了访谈，眼科检查包括使用早期治疗糖尿病视网膜病变研究（ETDRS）图表、光谱域光学相干断层扫描（SD-OCT）、裂隙灯检查和眼底照相进行视力评估。此外，抽取静脉血并制备用于流式细胞术。对细胞进行门控并测量表面标志物。测量单核细胞和B淋巴细胞与亚群的相对量，以及选定的表面标志物。两组在年龄、吸烟史、体重指数、体力活动或C反应蛋白（CRP）方面没有显著差异。新生血管性AMD组的总单核细胞（占所有白细胞的百分比）（中位数5.5%）低于对照组（6.5%; P值：0.028）。与对照组的100%相比，AMD患者的分化簇11b（CD 11b）阳性的中间单核细胞百分比（99.4%）较低（P值：0.032）。我们观察到较低数量的单核细胞，这表明在新生血管性AMD患者中迁移穿过内皮壁的能力可能受损。这些微妙的变化可能会导致疾病发展过程中巨噬细胞向视网膜中募集的不平衡。
Individuals with neovascular age-related macular degeneration (AMD) have altered number and distribution of retinal macrophages and show changes in circulating antibodies. We wanted to investigate the corresponding precursors, with subpopulations. We therefore measured monocyte and B-lymphocyte populations in individuals with neovascular AMD. This was an observational case–control study. A total of 31 individuals with neovascular AMD and 30 healthy age-matched controls were included. Patients and controls were interviewed, and ophthalmological examination included visual acuity assessment using the Early Treatment Diabetic Retinopathy Study (ETDRS) chart, spectral domain optical coherence tomography (SD-OCT), slit-lamp examination and fundus photography. Moreover, venous blood was drawn and prepared for flow cytometry. Cells were gated and measured for surface markers. Relative amounts of monocytes and B-lymphocytes with subsets, as well as selected surface markers, were measured. The two groups did not significantly differ in age, smoking history, body mass index, physical activity or C-reactive protein (CRP). Total monocytes (percentage of all leukocytes) were lower in the neovascular AMD group (median 5.5%) compared with the level in the control group (6.5%; P-value: 0.028). The percentage of intermediate monocytes positive for cluster of differentiation 11b (CD11b) was lower for AMD patients (99.4%) compared with 100% for the control group (P-value: 0.032). We observed lower numbers of monocytes, which show a potentially impaired ability to migrate across the endothelial wall in patients with neovascular AMD. These subtle changes could potentially lead to an imbalance in the recruitment of macrophages into the retina during disease development.