BACTERIAL BIOLUMINESCENCE - ISOLATION AND GENETIC-ANALYSIS OF FUNCTIONS FROM VIBRIO FISCHERI
BACTERIAL BIOLUMINESCENCE - ISOLATION AND GENETIC-ANALYSIS OF FUNCTIONS FROM VIBRIO FISCHERI
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DOI：
10.1016/0092-8674(83)90063-6
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发表时间：
1983-01-01
期刊：
影响因子：
64.5
通讯作者：
SILVERMAN, M
中科院分区：
文献类型：
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作者：
ENGEBRECHT, J;NEALSON, K;SILVERMAN, M
在含有来自海洋细菌费氏弧菌的DNA的杂交质粒的克隆文库中发现了产生光的重组大肠杆菌。所有的发光克隆有一个16 kb的插入编码的光反应和调控功能的发光表型（勒克斯）的表达所必需的酶活性[pH7.5]。由转座子Tn 5和mini-Mu产生的突变体用于定义Lux功能，并确定Lux区域的遗传组织。调节和酶的功能被分配到2个勒克斯操纵子的区域。通过转座子min-Mu上的lacZ基因与靶基因之间的转录融合，可以在不产生光的情况下测量lux操纵子的表达。lux操纵子的转录方向是从融合质粒中的mini-Mu插入的方向推断的。一个lux操纵子的转录诱导需要由该操纵子编码的功能（自动调节）。从这些和其他调节关系中，提出了光产生遗传控制的模型。
Recombinant Escherichia coli that produce light were found in a clone library of hybrid plasmids containing DNA from the marine bacterium V. fischeri. All luminescent clones had a 16 kb [kilobase] insert that encoded enzymatic activities for the light reaction and regulatory functions necessary for expression of the luminescence phenotype (Lux). Mutants generated by transposons Tn5 and mini-Mu were used to define Lux functions, and to determine the genetic organization of the lux region. Regulatory and enzymatic functions were assigned to regions of 2 lux operons. With transcriptional fusions between the lacZ gene on transposon min-Mu and the target gene, expression of lux operons could be measured in the absence of light production. The direction of transcription of lux operons was deduced from the orientation of mini-Mu insertions in the fusion plasmids. Induction of transcription of one lux operon required a function encoded by that operon (autoregulation). From these and other regulatory relationships, a model for genetic control of light production is proposed.