Mitogenomic analyses from ancient DNA
Mitogenomic analyses from ancient DNA
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DOI：
10.1016/j.ympev.2012.06.002
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发表时间：
2013-11-01
影响因子：
4.1
通讯作者：
Hofreiter, Michael
中科院分区：
文献类型：
--
作者：
Paijmans, Johanna L. A.;Gilbert, M. Thomas P.;Hofreiter, Michael
古DNA分析在保护遗传学、系统发育和种群遗传学分析中发挥着越来越重要的作用，因为它可以将灭绝物种纳入DNA序列树，并增加种群遗传学研究的时间深度。多年来，这些类型的DNA分析（无论是使用现代还是古代DNA）主要限于分析线粒体基因组的短片段。然而，由于过去十年中的许多技术进步，越来越多的研究探索了完整线粒体基因组序列（有丝分裂基因组）的力量。这些研究最初仅限于对现存生物的分析，但DNA测序技术和与降解DNA相关的一般方法学方面的发展导致完整的有丝分裂基因组在古代DNA研究中也越来越受欢迎。到目前为止，已经从20多个物种中获得了至少124个部分或完全组装的有丝分裂基因组，并且鉴于测序技术的快速进步，这一数字在未来可能会急剧增加。通过分析完整的有丝分裂基因组所提供的更多信息内容，在灭绝物种的系统发育位置以及解决灭绝和现存物种的群体遗传学问题方面取得了重大进展。(C)2012 Elsevier Inc. All rights reserved.
The analysis of ancient DNA is playing an increasingly important role in conservation genetic, phylogenetic and population genetic analyses, as it allows incorporating extinct species into DNA sequence trees and adds time depth to population genetics studies. For many years, these types of DNA analyses (whether using modern or ancient DNA) were largely restricted to the analysis of short fragments of the mitochondria] genome. However, due to many technological advances during the past decade, a growing number of studies have explored the power of complete mitochondrial genome sequences (mitogenomes). Such studies were initially limited to analyses of extant organisms, but developments in both DNA sequencing technologies and general methodological aspects related to working with degraded DNA have resulted in complete mitogenomes becoming increasingly popular for ancient DNA studies as well. To date, at least 124 partially or fully assembled mitogenomes from more than 20 species have been obtained, and, given the rapid progress in sequencing technology, this number is likely to dramatically increase in the future. The increased information content offered by analysing full mitogenomes has yielded major progress with regard to both the phylogenetic positions of extinct species, as well as resolving population genetics questions in both extinct and extant species. (C) 2012 Elsevier Inc. All rights reserved.