Identification of a Ly-6 superfamily gene expressed in lateral line neuromasts in zebrafish
Identification of a Ly-6 superfamily gene expressed in lateral line neuromasts in zebrafish
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DOI：
10.1007/s00427-015-0487-2
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发表时间：
2015-01
影响因子：
2.4
通讯作者：
Dongrui Ji;Lingyi Li;Shicui Zhang;Hongyan Li
中科院分区：
文献类型：
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作者：
Dongrui Ji;Lingyi Li;Shicui Zhang;Hongyan Li
斑马鱼中已鉴定出淋巴细胞抗原 6 (Ly-6) 超家族成员，但这些 Ly-6 基因的表达和功能仍然很大程度上未知。后侧线 (pLL) 系统是由 pLL 原基 (pLLp) 迁移产生的。趋化因子信号、Notch、Wnt 和成纤维细胞生长因子 (FGF) 信号调节 pLLp 细胞的迁移和神经丘的形成。然而，神经丘沉积的机制仍有待探索。鉴定 pLLp 中表达的新基因肯定有助于研究这一过程。在这里，我们鉴定了一种称为神经瘤表达的gpi锚定淋巴细胞抗原6（negaly6）的Ly-6基因，该基因在神经瘤中特异性表达。实时定量PCR（qRT-PCR）分析显示negaly6在24hpf时开始表达，整体原位杂交分析表明negaly6在pLLp尾部和成熟神经丘中高表达。此外，negaly6的表达被FGF信号拮抗剂抑制，但不被Wnt信号激动剂或拮抗剂抑制。总的来说，这些数据表明negaly6可能通过FGF信号通路与神经丘沉积的调节有关。
Lymphocyte antigen-6 (Ly-6) superfamily members have been identified in zebrafish, but the expression and function of these Ly-6 genes remain largely unknown. Posterior lateral line (pLL) system is produced by migrating pLL primordium (pLLp). Chemokine signaling, Notch, Wnt, and fibroblast growth factor (FGF) signaling regulate migration of pLLp cells and formation of neuromasts. However, the mechanism of neuromast deposition remains to be explored. Identification of novel genes expressed in pLLp will certainly help the study of such a process. Here we identified a Ly-6 gene calledneuromast-expressed gpi-anchored lymphocyte antigen-6(negaly6), which was specifically expressed in neuromast. Quantitative real-time PCR (qRT-PCR) analysis showed thatnegaly6started to be expressed at 24 hpf, and whole-mount in situ hybridization analysis indicated thatnegaly6was highly expressed in the trailing zone of pLLp and mature neuromast. Furthermore,negaly6expression was inhibited by FGF signaling antagonist but not by Wnt signaling agonist or antagonist. Collectively, these data indicate thatnegaly6may be associated with the regulation of neuromast deposition via FGF signaling pathway.