Protein kinase C-mediated phosphorylation of Kv beta 2 in adult rat brain.
Protein kinase C-mediated phosphorylation of Kv beta 2 in adult rat brain.
复制标题
成年大鼠脑中蛋白激酶 C 介导的 Kv beta 2 磷酸化。
DOI：
10.1023/b:nere.0000042215.92952.3d
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发表时间：
2004
影响因子：
4.4
通讯作者：
Prystowsky,MichaelB
中科院分区：
文献类型：
--
作者：
Wang,Xintao;Zhang,Jie;Berkowski,StanM;Knowleg,Heather;Chandramouly,AB;Downens,Martha;Prystowsky,MichaelB
研究了不同蛋白激酶对Kvβ2磷酸化的影响。蛋白激酶A催化亚基（PKA-CS）对重组Kvβ2 （rKvβ2）的磷酸化作用最大，蛋白激酶C催化亚基（PKC-CS）的磷酸化作用有限，酪蛋白激酶II （CKII）的磷酸化作用未检测到。来自成年大鼠脑裂解液的蛋白激酶(s)可磷酸化rKvβ2和内源性Kvβ。PKA抑制剂PKI 6-22完全抑制PKA介导的rKvβ2的磷酸化，但对大鼠脑中的激酶活性的抑制作用很小。阻断PKCα、PKCβ、PKCγ、PKCδ和PKCζ活性的抑制剂Gö 6983可以抑制大鼠脑激酶对rKvβ2的磷酸化，而阻断PKCα和PKCβΙ活性的抑制剂Gö 6976则没有抑制作用。Gö 6983抑制活性的剂量-反应分析表明，至少有两种PKC同工酶参与了大鼠脑中的激酶活性。Τhus，虽然PKA是体外磷酸化rKvβ2最活跃的蛋白激酶，但大鼠脑中Kvβ2的磷酸化主要是由PKC同工酶介导的。
The phosphorylation of Kvβ2 was investigated by different protein kinases. Protein kinase A catalytic subunit (PKA-CS) yielded the greatest phosphorylation of recombinant Kvβ2 (rKvβ2), with limited phosphorylation by protein kinase C catalytic subunit (PKC-CS) and no detectable phosphorylation by casein kinase II (CKII). Protein kinase(s) from adult rat brain lysate phosphorylated both rKvβ2 and endogenous Kvβ. The PKA inhibitor, PKI 6-22, fully inhibited PKA-mediated phophorylation of rKvβ2 yet showed minimal inhibition of kinase activity present in rat brain. The inhibitor Gö 6983, that blocks PKCα, PKCβ, PKCγ, PKCδ and PKCζ activities, inhibited rKvβ2 phosphorylation by rat brain kinases, with no inhibition by Gö 6976 which blocks PKCα and PKCβΙ activities. Dose-response analysis of Gö 6983 inhibitory activity indicates that at least two PKC isozymes account for the kinase activity present in rat brain. Τhus, while PKA was the most active protein kinase to phosphorylate rKvβ2 in vitro, Kvβ2 phosphorylation in the rat brain is mainly mediated by PKC isozymes.