Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
Generation of a genetically modified human embryonic stem cells expressing fluorescence tagged ATOX1
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DOI：
10.1016/j.scr.2019.101631
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发表时间：
2019-12-01
影响因子：
1.2
通讯作者：
Chen, Tai-Yen
中科院分区：
文献类型：
--
作者：
Wen, Meng-Hsuan;Xie, Xihong;Chen, Tai-Yen
ATOX1 is a copper chaperone involved in intracellular copper homeostasis, cell proliferation, and tumor progression. To investigate the physiologically relevant molecular mechanism of ATOX1 by using imaging-based approaches, we genetically modified ATOX1 in H1 hESCs to express mCherry-ATOX1 fusion protein under endogenous regulatory machinery. The fluorescence engineered hESC clone maintains characteristic stem cell features and can differentiate to all three germ layers, serving as a unique tool to dissect the role of ATOX1 in various cellular processes.
