Oxygen-generating hybrid nanoparticles to enhance fluorescent/photoacoustic/ultrasound imaging guided tumor photodynamic therapy
Oxygen-generating hybrid nanoparticles to enhance fluorescent/photoacoustic/ultrasound imaging guided tumor photodynamic therapy
复制标题
产氧混合纳米颗粒可增强荧光/光声/超声成像引导的肿瘤光动力治疗。
DOI：
10.1016/j.biomaterials.2016.10.030
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发表时间：
2017-01-01
期刊：
影响因子：
14
通讯作者：
Zhu, Lei
中科院分区：
文献类型：
--
作者：
Gao, Shi;Wang, Guohao;Zhu, Lei
光动力疗法（PDT）是一种很有前途的肿瘤治疗方式，它可以通过光敏剂将氧转化为细胞毒性单线态氧（SO）来消融肿瘤的生长。然而，肿瘤发展过程中不受控制的癌细胞增殖和PDT过程中的耗氧量往往导致肿瘤内氧水平不足，进而影响PDT效率。我们通过将二氧化锰纳米颗粒（MnO2 NP）包裹在吲哚菁绿（ICG）修饰的透明质酸纳米颗粒（HANP）中来设计一种产氧PDT纳米复合物，以克服这一限制。由于ICG-HANP/MnO2 （IHM）纳米复合物具有优异的荧光和光声特性，我们通过荧光成像和光声成像监测了ICG-HANP/MnO2 （IHM）纳米复合物静脉注射至SCC7肿瘤小鼠模型后的肿瘤积累情况。荧光和光声信号均较高，并在注射后6 h达到峰值（肿瘤-肌肉比值：荧光成像4.03 +/- 0.36，光声成像2.93 +/- 0.13）。此外，超声成像证实，由于MnO2 NP对H2O2的高反应性，不利于肿瘤细胞的代谢，肿瘤中氧含量比未经IHM处理的高2.25 +/- 0.07倍。激光照射后，与icg - hanp治疗组相比，IHM治疗组观察到明显的肿瘤生长抑制，这归因于IHM对PDT有益的产氧特性。预计IHM的设计将为提高临床PDT疗效提供另一种途径，并将在癌症治疗中得到广泛应用。(C) 2016 Elsevier Ltd.版权所有。
Photodynamic therapy (PDT) is a promising tumor treatment modality that can convert oxygen into cytotoxic singlet oxygen (SO) via photosensitizer to ablate tumor growth. However, the uncontrolled cancer cell proliferation during tumor development and the oxygen consumption during PDT always result in an insufficient oxygen level in tumors, which can adversely affect the PDT efficiency in turn. We designed an oxygen-generating PDT nanocomplex by encapsulating a manganese dioxide nanoparticle (MnO2 NP) in an indocyanine green (ICG) modified hyaluronic acid nanoparticle (HANP) to overcome this limitation. Because of the excellent fluorescent and photoacoustic properties, the tumor accumulation of the ICG-HANP/MnO2 (IHM) nanocomplex was monitored by fluorescent imaging and photoacoustic imaging after intravenous administration into the SCC7 tumor-bearing mouse model. Both high fluorescent and photoacoustic signals were detected and found peak at 6 h post-injection (tumor-muscle ratio: 4.03 +/- 0.36 for fluorescent imaging and 2.93 +/- 0.13 for photoacoustic imaging). In addition, due to the high reactivity of MnO2 NP to H2O2, an unfavorable tumor cell metabolic, the oxygen content in the tumor is elevated 2.25 +/- 0.07 times compared to that without IHM treatment as ultrasound imaging confirmed. After laser irradiation, significant tumor growth inhibition was observed in the IHM-treated group compared to the ICG-HANP-treated group, attributed to the beneficial oxygen-generating property of IHM for PDT. It is expected that the design of IHM will provide an alternative way of improving clinical PDT efficacy and will be widely applied in cancer theranostics. (C) 2016 Elsevier Ltd. All rights reserved.