Synchrotron radiation coronary microangiography for morphometric and physiological evaluation of myocardial neovascularization induced by endothelial progenitor cell transplantation
Synchrotron radiation coronary microangiography for morphometric and physiological evaluation of myocardial neovascularization induced by endothelial progenitor cell transplantation
复制标题
DOI：
10.1161/atvbaha.106.137141
复制
发表时间：
2007-06-01
影响因子：
8.7
通讯作者：
Asahara, Takayuki
中科院分区：
文献类型：
--
作者：
Iwasaki, Hiroto;Fukushima, Kazuhito;Asahara, Takayuki
背景-干细胞移植(SCTx)对小动物心肌新生血管的治疗效果是通过组织学毛细血管密度来评估的。然而，常规血管造影很难获得侧支循环形成的影像证据。方法和结果-从严重肢体缺血患者的外周血中分离CD34+和CD34-细胞。结扎裸鼠左前降支后，将PBS、CD34-细胞或CD34+细胞移植到心肌内。使用第三代同步辐射微血管造影术(SRM)对治疗后5天和28天的体外跳动心脏进行冠状动脉造影术，该造影术有可能显示直径小至20am的血管。在硝普钠(SNP)治疗前后分别进行SRM检查，观察各时间点的血管生理变化。大多数侧支血管直径为20~120am，在常规血管造影中明显看不到大小。CD34+细胞组在SNP前、后第28天的Rtrop评分均显著高于其他组(P
Background-Therapeutic effect of stem cell transplantation (SCTx) for myocardial neovascularization has been evaluated by histological capillary density in small animals. However, it has been technically difficult to obtain imaging evidence of collateral formation by conventional angiography.Methods and Results-Peripheral blood CD34+ and CD34- cells were isolated from patients with critical limb ischemia. PBS, CD34- cells, or CD34+ cells were intramyocafdially transplanted after ligating LAD of nude rats. Coronary angiography of ex vivo beating hearts 5 and 28 days after the treatment was performed using the third generation synchrotron radiation microangiography (SRM), which has potential to visualize vessels as small as 20 Am in diameter. The SRM was performed pre and post sodium nitroprusside (SNP) to examine vascular physiology at each time point. Diameter of most collateral vessels was 20 to 120 Am, apparently invisible size in conventional angiography. Rentrop scores at day 28 pre and post SNP were significantly greater in CD34+ cell group than other groups (P