A comparison of diagnostic techniques for postpartum endometritis in dairy cattle
A comparison of diagnostic techniques for postpartum endometritis in dairy cattle
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DOI：
10.1016/j.theriogenology.2007.12.005
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发表时间：
2008-04-01
期刊：
影响因子：
2.8
通讯作者：
Palmer, C. W.
中科院分区：
文献类型：
--
作者：
Barlund, C. S.;Carruthers, T. D.;Palmer, C. W.
采用5种诊断技术对8个商业奶牛群的荷斯坦奶牛（n = 22 1）产后28 ~ 41天的子宫内膜炎进行了检查：（1）阴道镜检查;（2）子宫液量的超声评估;（3）子宫内膜厚度的超声评估;（4）细胞刷收集的子宫内膜细胞学检查;（5）子宫灌洗收集的子宫内膜细胞学检查。采用一致性相关分析评价细胞刷和灌洗细胞学检查的可靠性。发现细胞刷细胞学具有最大的观察者内重复性（细胞刷，p（c）= 0.85 vs灌洗，p（c）= 0.76），并被选为参考诊断试验。可获得189头奶牛产后150天的妊娠数据。生存分析用于确定与妊娠时间相关的多形核细胞的最低百分比。以150 d妊娠状态和细胞刷细胞学为诊断标准，细胞刷细胞学鉴定出PMNs> 8%的奶牛150 d未妊娠的风险是PMNs <8%的奶牛150 d未妊娠的风险的1.9倍（P = 0.04）。189头奶牛中有21头奶牛（11.1%）的PMN> 8%，被认为是肠炎阳性。患子宫内膜炎的奶牛首次受孕率降低17.9%（P = 0.03），中位开放日增加24天（P = 0.04）。所有五种诊断试验相对于150天妊娠状态的敏感性范围为7.1%至14.3%，特异性为84.0%至93.3%。相对于细胞刷细胞学检查，其敏感性和特异性分别为：阴道镜检查（53.9%，95.4%）;灌洗液细胞学检查（92.3%，93.9%）;子宫液超声评估（30.8%，92.8%）;子宫内膜厚度超声评估（3.9%，89.2%）。子宫内膜炎损害生殖能力。细胞刷细胞学检查是诊断牛盲肠炎最可靠的方法。(c)2008年爱思唯尔公司所有的战斗保留。
Holstein cows (n = 22 1) from eight commercial dairy herds were examined for endometritis between 28 and 41 days postpartum using 5 diagnostic techniques: (1) vaginoscopy; (2) ultrasonographic assessment of uterine fluid volume; (3) ultrasonographic assessment of endometrial thickness; (4) endometrial cytology collected by cytobrush; and (5) endometrial cytology collected by uterine lavage. Concordance correlation was used to evaluate the reliability of cytobrush and lavage cytology. Cytobrush cytology was found to have the greatest intraobserver repeatability (cytobrush, p(c) = 0.85 versus lavage, p(c) = 0.76) and was chosen as the reference diagnostic test. Pregnancy data at 150 days postpartum was available for 189 cows. Survival analysis was used to determine the lowest percentage of polymorphonuclear cells associated with time to pregnancy. The sensitivity and specificity of the diagnostic techniques was determined using pregnancy status at 150 days and cytobrush cytology as the diagnostic standards.The risk of non-pregnancy at 150 days was 1.9 times higher in cows with more than 8% PMNs identified using cytobrush cytology than in cows with less than 8% PMNs (P = 0.04). Twenty-one cows of 189 cows (11.1%) had > 8% PMNs and were considered to be positive for endometritis. Cows with endometritis had a 17.9% lower first service conception rate (P = 0.03) and a 24-day increase in median days open (P = 0.04). The sensitivities of all five diagnostic tests relative to 150-day pregnancy status ranged from 7.1 to 14.3% and the specificities from 84.0 to 93.3%. Relative to cytobrush cytology, the respective sensitivity and specificity values are as follows: vaginoscopy (53.9%, 95.4%); lavage cytology (92.3%, 93.9%); ultrasonographic assessment of uterine fluid (30.8%, 92.8%); and ultrasonographic assessment of endometrial thickness (3.9%, 89.2%). Endometritis impaired reproductive performance. Cytobrush cytology was the most reliable method of diagnosing endometritis in cattle. (c) 2008 Elsevier Inc. All fights reserved.