Facile Synthesis of Mg2 -Doped Carbon Dots as Novel Biomaterial Inducing Cell Osteoblastic Differentiation
Facile Synthesis of Mg2 -Doped Carbon Dots as Novel Biomaterial Inducing Cell Osteoblastic Differentiation
复制标题
轻松合成掺镁碳点作为诱导细胞成骨细胞分化的新型生物材料
DOI：
10.1002/ppsc.201800315
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发表时间：
2019
影响因子：
2.7
通讯作者：
Yang Bai
中科院分区：
文献类型：
--
作者：
Yang Mingxi;Meng Yang;Liu Junjun;Yu Weixian;Yang Bai
碳量子点作为一种新兴的低毒性荧光纳米材料，已广泛应用于各种生物相关领域。然而，关于它们引导成骨分化的研究却很少，这对骨质疏松症的治疗和骨再生具有重要意义。在此，首次通过一步水热法从金属葡萄糖酸盐容易地合成了一种新的Mg 2+掺杂的CD。CD可以作为进入细胞的Mg 2+离子的纳米载体，并且生物必需的金属离子随后通过提高碱性磷酸酶（ALP）活性和上调相关mRNA表达来刺激成骨细胞分化。值得注意的是，与Mg-CD相比，原材料在成骨细胞分化方面的性能几乎可以忽略不计，这是由于CD的超小尺寸和细胞的有效吸收。此外，受益于荧光特性，Mg-CD也可以用作细胞标记剂。这项工作提出了一种新的策略来合成多功能金属离子掺杂的CD，这可能在骨丢失治疗中具有很大的潜力。
Carbon dots (CDs), as an emerging fluorescent nanomaterial with low toxicity, has been widely applied in various bio‐related fields. However, investigations on their capabilities in guiding osteogenic differentiation are rarely seen, which has great significance in osteoporosis therapy and bone regeneration. Herein, for the first time, a new kind of Mg2+‐doped CDs is facilely synthesized through a one‐step hydrothermal method from metal gluconate salts. The CDs can serve as nanocarrier of Mg2+ions entering into cells, and the bioessential metal ions subsequently stimulate osteoblastic differentiation by improving alkaline phosphatase (ALP) activity and upregulation related mRNA expression. Noteworthy, the raw material has almost negligible performance on osteoblastic differentiation compared to Mg‐CDs, which is due to the ultrasmall sizes of CDs and the efficient uptake by cells. Moreover, benefitting from the fluorescence properties, Mg‐CDs can also be applied as cell labeling agents. This work proposes a new strategy to synthesize multifunctional metal ion‐doped CDs, which might had great potential in serving as promising nanodrugs for bone loss therapy.