Identification of two quenching sites active in the regulation of photosynthetic light-harvesting studied by time-resolved fluorescence
Identification of two quenching sites active in the regulation of photosynthetic light-harvesting studied by time-resolved fluorescence
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DOI：
10.1016/j.cplett.2009.10.085
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发表时间：
2009-12-01
影响因子：
2.8
通讯作者：
Jahns, Peter
中科院分区：
文献类型：
--
作者：
Holzwarth, Alfred R.;Miloslavina, Yuliya;Jahns, Peter
光捕获的调节（称为非光化学猝灭，NPQ）是植物中活跃的重要光保护机制。总 NPQ 取决于 PsbS（一种 pH 感应蛋白）和叶黄素类胡萝卜素玉米黄质 (Zx) 的作用。在完整的叶子上使用超快荧光，我们证明了体内两个独立的 NPQ 猝灭位点，它们不同地依赖于 PsbS 和 Zx 的作用。第一个位点形成于 PS II 功能独立的主要光捕获复合体中，并且严格依赖于 PsbS。第二个位点位于光系统 (PS) II 的小触角中，猝灭取决于 Zx 的存在。 (C) 2009 Elsevier B.V. 保留所有权利。
The regulation of light-harvesting (called non-photochemical quenching, NPQ) is an essential photoprotective mechanism active in plants. Total NPQ is dependent on PsbS, a pH-sensing protein, and on the action of the xanthophyll carotenoid zeaxanthin (Zx). Using ultrafast fluorescence on intact leaves we demonstrate two independent NPQ quenching sites in vivo which depend differently on the actions of PsbS and Zx. The first site is formed in the functionally detached major light-harvesting complex of PS II and depends strictly on PsbS. The second site is in the minor antennae of photosystem (PS) II and quenching depends on the presence of Zx. (C) 2009 Elsevier B.V. All rights reserved.