Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering
Human umbilical cord mesenchymal stem cells: Osteogenesis in vivo as seed cells for bone tissue engineering
复制标题
人脐带间充质干细胞：作为骨组织工程种子细胞的体内成骨作用
DOI：
10.1002/jbm.a.32186
复制
发表时间：
2009-10-01
影响因子：
4.9
通讯作者：
Zhong, Yanfeng
中科院分区：
文献类型：
--
作者：
Diao, Yinze;Ma, Qingjun;Zhong, Yanfeng
间充质干细胞（MSC）是骨组织工程的理想种子细胞。然而，利用患者骨髓间充质干细胞构建人工骨的自体移植策略存在固有的缺陷。在这项研究中，从人脐带中分离出类间充质干细胞，并在体外进行扩增。流式细胞分析显示，第四代细胞的 CD29、CD44、CD71、CD73、CD90 和 CD105 呈阳性，而 CD14、CD34、CD45 和 CD117 呈阴性。此外，这些细胞表达 HLA-A、B、C (MHC-I)，但不表达 HLA-DP、DQ、DR (MHC-II) 或共刺激分子，例如 CD80 和 CD86。在特定的诱导培养基中孵育 3 周后，培养的细胞被证明具有在体外分化成脂肪形成、成骨或软骨形成谱系的潜力。脐带间充质干细胞（UC-MSCs）装载仿生人工骨支架材料，然后皮下植入Balb/c裸鼠背部4至12周。我们的结果表明，装载支架的UC-MSC表现出成骨分化能力，从而导致体内人源性成骨。作为骨组织工程种子细胞的现成来源，UC-MSCs应该具有广阔的应用前景。 (C) 2008 Wiley periodicals, Inc. J Biomed Mater Res 91A: 123-131, 2009
Mesenchymal stem cells (MSCs) are ideal seed cells for bone tissue engineering. However, intrinsic deficiencies exist for the autologous transplantation strategy of constructing artificial bone with MSCs derived from bone marrow of patients. In this study, MSCs-like cells were isolated from human umbilical cords and were expanded in vitro. Flow cytometric analysis revealed that cells from the fourth passage were positive for CD29, CD44, CD71, CD73, CD90, and CD105 whereas they were negative for CD14, CD34, CD45, and CD117. Furthermore, these cells expressed HLA-A, B, C (MHC-I), but not HLA-DP, DQ, DR (MHC-II), or costimulatory molecules such as CD80 and CD86. Following incubation in specific inductive media for 3 weeks, cultured cells were shown to possess potential to differentiate into adipogenic, osteogenic or chondrogenic lineages in vitro. The umbilical cord-derived MSCs (UC-MSCs) were loaded with a biomimetic artificial bone scaffold material before being implanted subcutaneously in the back of Balb/c nude mice for four to twelve weeks. Our results revealed that UC-MSCs loaded with the scaffold displayed capacity of osteogenic differentiation leading to osteogenesis with human origin in vivo. As a readily available source of seed cells for bone tissue engineering, UC-MSCs should have broad application prospects. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 123-131, 2009