Expression of monocyte chemoattractant protein (MCP-1) and nitric oxide synthase-2 following cerebral trauma
Expression of monocyte chemoattractant protein (MCP-1) and nitric oxide synthase-2 following cerebral trauma
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DOI：
10.1007/s004010050770
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发表时间：
1998-01-01
影响因子：
12.7
通讯作者：
Murphy, S
中科院分区：
文献类型：
--
作者：
Grzybicki, D;Moore, SA;Murphy, S
脑创伤性损伤启动多个相互关联的过程，涉及实质、血管和浸润性炎性细胞。一氧化氮（NO）和趋化因子已牵连作为调节中枢神经系统IR？损伤反应：小鼠大脑皮层低温损伤后，用逆转录聚合酶链反应（RT-PCR）检测损伤后12 h同侧脑组织中NO合酶（NOS）-2 mRNA的表达，并持续2周。虽然mRNA也检测到对侧，表达的时间过程较短（1周）。免疫组化结果显示，损伤后12 h，NOS-2蛋白在同侧浸润的炎性细胞中表达。星形胶质细胞在损伤后24 ~ 72 h表达NOS-2。损伤侧单核细胞趋化蛋白（MCP-1）mRNA表达于6 h达高峰，持续24 h，48 h下降。在未损伤侧，MCP-1 mRNA的表达程度要低得多，并在24 h时下降。MCP-1的上调是相对特异的，因为编码IP-10的密切相关的mRNA没有显著增加。这些发现暗示了NOS-2和MCP-1作为低温脑创伤后细胞事件的潜在调节因子的作用。
Traumatic injury to the brain initiates multiple interrelated processes that involve parenchymal, vascular, and infiltrating inflammatory cells. Nitric oxide (NO) and chemokines have been implicated as regulators of the central nervous system ir?jury response, Following a cryogenic lesion of the cerebral cortex in mice, mRNA for NO synthase (NOS)-2 was detected by reverse transcriptase polymerase chain reaction ipsilaterally 12 h after injury and persisted for 2 weeks. While mRNA was also detected contralaterally, the time course of expression was shorter (1 week). By immunohistochemistry, NOS-2 protein was initially detected ipsilaterally 12 h after injury in infiltrating inflammatory cells. Astroglial cells expressed NOS-2 from 24 to 72 h after injury. The expression of monocyte chemoattractant protein (MCP-1) mRNA peaked at 6 h on the lesion side, remained for 24 h and then declined by 48 h. On the unlesioned side, MCP-1 mRNA was expressed to a much lesser extent and had declined by 24 h. The up-regulation of MCP-1 was relatively specific as a closely related mRNA encoding IP-10 was not significantly increased. These findings implicate a role for NOS-2 and MCP-I as potential regulators of cellular events following cryogenic cerebral trauma.