SOMATIC MUTATION AND THE MATURATION OF IMMUNE-RESPONSE TO 2-PHENYL OXAZOLONE
SOMATIC MUTATION AND THE MATURATION OF IMMUNE-RESPONSE TO 2-PHENYL OXAZOLONE
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DOI：
10.1038/312271a0
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发表时间：
1984-01-01
期刊：
影响因子：
64.8
通讯作者：
MILSTEIN, C
中科院分区：
文献类型：
--
作者：
GRIFFITHS, GM;BEREK, C;MILSTEIN, C
对免疫反应发展的研究表明，表达的抗体特异性受抗原的强烈影响（参考文献 1 中的综述）。体现这种影响的一种方式是免疫后抗体对抗原的亲和力随着时间的推移逐渐增加。这种现象被称为免疫反应的“成熟”，一定是由于正在合成的抗体结构发生了变化。然而，所涉及的变化的确切性质以及产生这些变化的遗传机制尚未明确定义。我们现在通过特定杂交瘤的 mRNA 测序研究了对半抗原 2-苯基恶唑酮的免疫反应的成熟度。我们得出的结论是，种系编码基因的体细胞突变在免疫后随着时间的推移对恶唑酮的亲和力增加的抗体的产生中起着重要作用。
Studies on the development of the immune response suggest that the repertoire of expressed antibody specificities is strongly influenced by antigen (reviewed in ref. 1). One way in which this influence is manifested is by a progressive increase in the affinity of antibody for antigen with time after immunization. This phenomenon, termed the ‘maturation’ of the immune response, must be due to a change in the structure of the antibody being synthesized. However, the precise nature of the changes involved and the genetic mechanisms used to produce them have not been clearly defined. We have now investigated the maturation of the immune response to the hapten 2-phenyloxazolone by mRNA sequencing of specific hybridomas. We conclude that somatic mutation of germ-line encoded genes plays a major role in the generation of antibodies with increased affinity for oxazolone with time after immunization.