Effectiveness of plant-derived proanthocyanidins on demineralization on enamel and dentin under artificial cariogenic challenge.
Effectiveness of plant-derived proanthocyanidins on demineralization on enamel and dentin under artificial cariogenic challenge.
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DOI：
10.1590/1678-775720140304
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发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Shinohara MS
中科院分区：
文献类型：
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作者：
Silva AP;Gonçalves RS;Borges AF;Bedran-Russo AK;Shinohara MS
龋齿被认为是一种高发疾病，是公共卫生中的一个长期问题。原花青素（PAs）是最近旨在控制或治疗龋齿的研究的目标物质。本体外研究的目的是评估葡萄籽提取物在牙釉质和牙本质龋齿发生挑战下的治疗效果，以减少甚至防止龋齿的发生。从牛中切牙中获得牙釉质和牙本质块（6.0 × 6.0 mm），抛光，并通过表面显微硬度（SH）分析选择。各组按GC（对照）、GSE（葡萄籽提取物）、GF（氟- 1000 ppm）处理随机分为3组（n=15）。这些块每天进行6次pH循环，持续8天。在每日循环中，标本保存在缓冲溶液中。然后进行垂直硬度和表面硬度分析以及偏振光显微镜分析。采用方差分析和Fisher 's PLSD检验对平均值进行统计分析（p<0.05）。对于釉质SH， GF的硬度值最高。在牙本质中，GF硬度值最高，其次是GSE。关于横截面硬度值，所有组在牙本质和牙釉质上表现相似。用GSE和氟（GF）处理的样品在统计学上高于对照组。本体外实验数据表明，葡萄籽提取物对牙本质和牙釉质人工龋齿的脱矿均有抑制作用，但与氟化物相比，不同结构对脱矿的抑制程度不同。
Dental caries is considered a disease of high prevalence and a constant problem in public health. Proanthocyanidins (PAs) are substances that have been the target of recent studies aiming to control or treat caries. The aim of this in vitro study was to evaluate the efficacy of a treatment with grape seed extract, under cariogenic challenge, to minimize or even prevent the onset of caries in the enamel and dentin. Blocks of enamel and dentin (6.0x6.0 mm) were obtained from bovine central incisors, polished, and selected by analysis of surface microhardness (SH). The blocks were randomly divided into 3 groups (n=15), according to the following treatments: GC (control), GSE (grape seed extract), GF (fluoride – 1,000 ppm). The blocks were subjected to 6 daily pH cycles for 8 days. Within the daily cycling, the specimens were stored in buffered solution. The blocks were then analyzed for perpendicular and surface hardness and polarized light microscopy. The means were subjected to statistical analysis using the ANOVA and Fisher’s PLSD tests (p<0.05). For enamel SH, GF showed the highest hardness values. In the dentin, GF was also the one that showed higher hardness values, followed by GSE. Regarding the cross-sectional hardness values, all groups behaved similarly in both the enamel and dentin. The samples that were treated with GSE and fluoride (GF) showed statistically higher values than the control. Based on the data obtained in this in vitro study, it is suggested that grape seed extract inhibits demineralization of artificial carious lesions in both the enamel and dentin, but in a different scale in each structure and in a smaller scale when compared to fluoride.