DIFFERENTIAL ACTIVATION OF RAS GENES BY POINT MUTATION IN HUMAN COLON CANCER WITH METASTASES TO EITHER LUNG OR LIVER
DIFFERENTIAL ACTIVATION OF RAS GENES BY POINT MUTATION IN HUMAN COLON CANCER WITH METASTASES TO EITHER LUNG OR LIVER
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DOI：
10.1002/ijc.2910490613
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发表时间：
1991-12-02
影响因子：
6.4
通讯作者：
RODENHUIS, S
中科院分区：
文献类型：
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作者：
OUDEJANS, JJ;SLEBOS, RJC;RODENHUIS, S
为了研究ras癌基因激活在结肠癌扩散中的可能作用，我们确定了3组肿瘤中K-和N-ras密码子12、13和61的点突变：（A）接受过Dukes' B手术的患者的原发性肿瘤（早期）结肠癌，（B）来自经历孤立肺转移瘤切除术的患者的原发性肿瘤和转移瘤，和（C）原发性肿瘤和来自接受孤立肝转移瘤切除术的患者的转移瘤。在来自93名患者的129份样本中，54份（42%）在K-或N-ras点突变中呈阳性。大多数突变（89%）发生在K-ras基因。A组（n = 50）ras基因点突变16例（32%），其中K-ras 15例，N-ras 1例。B组23例中有13例（57%）ras基因点突变阳性，其中K-ras 10例，N-ras 3例。C组（n = 20）有10例（50%）K-ras基因第12位密码子点突变，而H-或N-ras基因无点突变。B组和C组中31例转移瘤的原发肿瘤可用于分析，15例含有ras点突变（48%）。并非所有的突变都存在于原发肿瘤和转移瘤中。在3例病例中，在转移灶中检测到突变，但在原发性肿瘤中未检测到突变，而在1例病例中，在原发性肿瘤中发现突变。
To study the possible role of ras oncogene activation in the dissemination of colon cancer, we determined point mutations in codons 12, 13 and 61 in K- and N-ras in 3 groups of tumors: (A) primary tumors of patients who had undergone surgery for Dukes' B (early-stage) colon cancer, (B) primary tumors and metastases from patients undergoing resection of isolated lung metastases and (C) primary tumors and metastases from patients undergoing resection of isolated liver metastases. In 129 samples from 93 patients, 54 (42%) were positive for point mutations in either K- or N-ras. Most mutations (89%) were found in the K-ras gene. In group A (n = 50) ras point mutations were detected in 16 cases (32%) (15 in K-ras and l in N-ras). Thirteen out of 23 cases in group B (57%) were positive for a ras point mutation: 10 in K-ras and 3 in N-ras. In group C (n = 20), point mutations in codon 12 of K-ras, but none in H- or N-ras, were found in 10 cases (50%). In 31 cases the primary tumors from the metastases in groups B and C were available for analysis and 15 contained a ras point mutation (48%). Not all mutations were present in both the primary tumor and the metastasis. In 3 instances, a mutation was detected in the metastasis but not in the primary tumor, whereas in 1 case a mutation was found in the primary tumor.