Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins
Design of bioelectronic interfaces by exploiting hinge-bending motions in proteins
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DOI：
10.1126/science.1062461
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发表时间：
2001-08-31
期刊：
影响因子：
56.9
通讯作者：
Hellinga, HW
中科院分区：
文献类型：
--
作者：
Benson, DE;Conrad, DW;Hellinga, HW
我们报告了一种灵活的策略，用于将配体结合事件转换为多种蛋白质的电化学响应。该方法利用配体介导的铰链弯曲运动，固有的细菌周质结合蛋白超家族，建立变构控制电极表面和氧化还原活性，Ru（II）标记的蛋白质之间的相互作用。这种方法允许开发基于蛋白质的生物电子接口，以响应不同的分析物。这些接口的家庭可以通过利用超家族内的天然结合多样性或通过重新设计单个蛋白质的特异性来产生。这些蛋白质可能具有许多医疗，环境和国防应用。
We report a flexible strategy for transducing ligand-binding events into electrochemical responses for a wide variety of proteins. The method exploits ligand-mediated hinge-bending motions, intrinsic to the bacterial periplasmic binding protein superfamily, to establish allosterically controlled interactions between electrode surfaces and redox-active, Ru(II)labeled proteins. This approach allows the development of protein-based bioelectronic interfaces that respond to a diverse set of analytes. Families of these interfaces can be generated either by exploiting natural binding diversity within the superfamily or by reengineering the specificity of individual proteins. These proteins may have numerous medical, environmental, and defense applications.