IMMUNOMODULATING ACTIONS OF CAROTENOIDS - ENHANCEMENT OF IN-VIVO AND IN-VITRO ANTIBODY-PRODUCTION TO T-DEPENDENT ANTIGENS
IMMUNOMODULATING ACTIONS OF CAROTENOIDS - ENHANCEMENT OF IN-VIVO AND IN-VITRO ANTIBODY-PRODUCTION TO T-DEPENDENT ANTIGENS
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DOI：
10.1080/01635589409514303
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发表时间：
1994-01-01
影响因子：
2.9
通讯作者：
TOMITA, Y
中科院分区：
文献类型：
--
作者：
JYONOUCHI, H;ZHANG, L;TOMITA, Y
在此之前，我们证明了在体外抗体（Ab）生产的增强响应T-依赖性抗原（TD-Ag）的虾青素，类胡萝卜素没有维生素A的活性。β-胡萝卜素，类胡萝卜素与维生素A的活动，和叶黄素，另一种类胡萝卜素没有维生素A的活动，在体外抗体生产的影响进行了检查与脾细胞从年轻和年老的B6小鼠。此外，在体内叶黄素，虾青素，β-胡萝卜素对抗体生产的影响进行了研究，在年轻和老年B6小鼠。叶黄素，而不是β-胡萝卜素，增强体外抗体生产响应TD-Ags。辅助性T细胞的耗竭阻止了叶黄素和虾青素对抗体产生的增强。在体内抗体生产响应TD-Ag显着增强叶黄素，虾青素，β-胡萝卜素。免疫球蛋白M-和G-分泌细胞的数量也增加，在体内与这些类胡萝卜素的管理时，小鼠与TD-Ag引发。在体内和体外的TD-Ags反应抗体生产显着低于年轻的B6小鼠在老年人。虾青素补充剂部分恢复减少在体内抗体生产响应TD-Ags在老B6小鼠。叶黄素和β-胡萝卜素也增强了体内抗体的生产响应TD-Ags在老B6小鼠，虽然在较小程度上比虾青素。然而，没有一个类胡萝卜素有影响在体内或体外抗体生产响应T-非依赖性antigen.These结果表明显着的免疫调节作用的类胡萝卜素的体液免疫应答TD-Ags和建议，类胡萝卜素补充可能是有益的，在老年动物的体液免疫应答的恢复。
Previously, we demonstrated an enhancement of in vitro antibody (Ab) production in response to T-dependent antigens (TD-Ag) by astaxanthin, a carotenoid without vitamin A activity. The effects of beta-carotene, a carotenoid with vitamin A activity, and lutein, another carotenoid without vitamin A activity, on in vitro Ab production were examined with spleen cells from young and old B6 mice. In addition, the in vivo effects of lutein, astaxanthin, and beta-carorene on Ab production were studied in young and old B6 mice.Lutein, but not beta-carotene, enhanced in vitro Ab production in response to TD-Ags. The depletion of T-helper cells prevented the enhancement of Ab production by lutein and astaxanthin. In vivo Ab production in response to TD-Ag was significantly enhanced by lutein, astaxanthin, and p-carotene. The numbers of immunoglobulin M- and G-secreting cells also increased in vivo with the administration of these carotenoids when mice were primed with TD-Ags. Antibody production in response to TD-Ags in vivo and in vitro was significantly lower in old than in young B6 mice. Astaxanthin supplements partially restored decreased in vivo Ab production in response to TD-Ags in old B6 mice. Lutein and beta-carotene also enhanced in vivo Ab production in response to TD-Ags in old B6 mice, although to a lesser extent than did astaxanthin. However, none of the carotenoids had an effect on in vivo or in vitro Ab production in response to T-independent antigen.These results indicate significant immunomodulating actions of carotenoids for humoral immune responses to TD-Ags and suggest that carotenoid supplementation may be beneficial in restoring humoral immune responses in older animals.