Significant changes in mitochondrial distribution in different pain models of mice
Significant changes in mitochondrial distribution in different pain models of mice
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不同疼痛模型小鼠线粒体分布的显着变化
DOI：
10.1016/j.mito.2013.03.007
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发表时间：
2013-07-01
期刊：
影响因子：
4.4
通讯作者：
Yang, Yan-Ling
中科院分区：
文献类型：
--
作者：
Guo, Bao-Lin;Sui, Bing-Dong;Yang, Yan-Ling
线粒体在炎症性和神经病理性疼痛的病理生理学中起重要作用，但其机制尚不清楚。到目前为止，还没有全面的研究来评估疼痛后线粒体动力学的变化。在本研究中，我们通过鞘内注射线粒体标记物Mitotracker Red(R)CM-H2XRox(Mito-Red)和共聚焦显微镜分析，检测了福尔马林诱导的急性炎症性疼痛、完全弗氏佐剂(CFA)诱导的持续性疼痛和神经损伤(SNI)诱导的神经病理性疼痛模型中线粒体的分布和亚细胞形态。结果表明，皮下注射福尔马林对急性期和紧张期脊髓背角内有丝分裂细胞的数量无明显影响，但在紧张期显著增加脊髓背角浅层(板层I-II)的簇型线粒体数量。不同的是，在持续性CFA和SNI神经病理性疼痛后，脊髓背角浅层和深层(板层)有丝分裂细胞的数量显著增加。此外，CFA和SNI均能显著增加脊髓背角浅层和深层的团簇型线粒体数量，减少颗粒型线粒体数量。因此，我们得出结论，线粒体分布异常与神经病理性疼痛和某些形式的炎症性疼痛有关。(C)2013年爱思唯尔公司和线粒体研究会。版权所有。
Mitochondria play an important role in pathophysiology of inflammatory and neuropathic pain but the mechanism is unclear. So far no comprehensive study exists that evaluates the changes of mitochondrial dynamics following the pain. In this study, we detected the mitochondrial distribution and subcellular morphology by using intrathecal injection of mitochondrial marker, Mitotracker Red (R) CM-H2XRox (Mito-Red) and confocal microscopic analysis in models of formalin-induced acute inflammatory pain, Complete Freund's Adjuvant (CFA)-induced persistent pain and spared nerve injury (SNI)-induced neuropathic pain. The results demonstrated that subcutaneous formalin injection did not affect the number of Mito-Red cells within the spinal dorsal horn at both acute and tonic phases, but significantly increased the number of cluster type mitochondria in superficial spinal dorsal horn (laminas I-II) at tonic phase. Differently, the number of Mito-Red cells significantly increased in superficial and deep spinal dorsal horn (laminas following persistent CFA and SNI neuropathic pain. Moreover, both CFA and SNI remarkably increased the number of cluster type mitochondria and decreased the number of granule type mitochondria, in both superficial and deep spinal dorsal horn. So we concluded that abnormal mitochondrial distribution contributes to neuropathic and some forms of inflammatory pain. (c) 2013 Elsevier B.V. and Mitochondria Research Society. All rights reserved.