Surface properties of mussel adhesive protein component films.
Surface properties of mussel adhesive protein component films.
复制标题
贻贝粘附蛋白成分膜的表面特性。
DOI：
10.1016/0142-9612(92)90150-m
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发表时间：
1992
期刊：
影响因子：
14
通讯作者：
Loomis,RE
中科院分区：
文献类型：
--
作者：
Olivieri,MP;Baier,RE;Loomis,RE
贻贝黏附蛋白(MAP)是蓝海贻贝(Mytilus Edulis)用来将动物附着在各种水下表面的黏附剂。它由75个→85重复十聚体单位组成，主序列为已报道的DNA序列这项研究确定并比较了十聚体单位、选定的片段和单个氨基酸成分的表面性质与完整的图谱准备。用多重衰减内反射红外光谱、椭偏仪和接触角分析研究了这些分子体系：(A)固体状态下在锗衬底上形成的薄膜；(B)溶液状态下的圆二色谱(CD)。对十聚体进行了广泛的分子模拟，综合利用了实验得出的数据。这些累积的半经验和经验结果表明，十聚体的构象与固体底物紧密地结合了l-DOPA和酪氨酸残基。该模型首次对理论数据和实验数据进行了合理的整合，得到了MAP图。在此基础上，对MAP的生物黏附特性提出了可能的解释。
Mussel adhesive protein (MAP) is the adhesive agent used by the blue sea mussel (Mytilus edulis) to attach the animal to various underwater surfaces. It is composed of 75→85 repeating decameric units with the reported primary sequence NH2-A(1)-K(2)-P(3)-S(4)-Y(5)-Hyp(6)-Hyp(7)-T(8)-DOPA(9)-K(10)-COOH. This study identifies and compares the surface properties of the decameric unit, selected fragments and individual amino acid constituents with the complete MAP preparation. These molecular systems were examined: (a) in the solid state as thin films formed on germanium substrata using multiple-attenuated-internal-reflectance infrared (MAIR-IR) spectroscopy, ellipsometry and contact angle analysis; and (b) in the solution state using circular dichroism (CD) spectroscopy. Extensive molecular modelling of the decamer was performed making integral use of the experimentally derived data. These cumulative semi-empirical and empirical results suggest a conformation for the decamer that closely associates thel-DOPA and tyrosine residues with the solid substratum. This model provides the first representation of MAP derived from a rational integration of theoretical and experimental data. On the basis of this model, a possible explanation for the bioadhesive properties of MAP is suggested.