Effects of Treatment on IgE Responses against Parasite Allergen-Like Proteins and Immunity to Reinfection in Childhood Schistosome and Hookworm Coinfections
Effects of Treatment on IgE Responses against Parasite Allergen-Like Proteins and Immunity to Reinfection in Childhood Schistosome and Hookworm Coinfections
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DOI：
10.1128/iai.00748-12
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发表时间：
2013-01-01
影响因子：
3.1
通讯作者：
Dunne, David W.
中科院分区：
文献类型：
--
作者：
de Moira, Angela Pinot;Jones, Frances M.;Dunne, David W.
人类对血吸虫病和钩虫感染的自然免疫与对寄生虫变应原样蛋白的IgE反应有关。由于这两种蠕虫经常同时感染同一个人，因此越来越多的人主张同时治疗这两种寄生虫。然而，已知这两种蠕虫都具有很强的免疫调节作用；因此，合并感染的人的免疫反应可能与单一感染的人不同。在这项研究中，我们测量了同时治疗合并感染曼氏血吸虫和钩虫的学童后，IgE、IgG1和IgG4对血吸虫和钩虫抗原的反应的变化，包括曼氏血吸虫被膜变应原样蛋白1(SmTAL1)、SmTAL2和美洲钩虫钩虫分泌蛋白2(Na-ASP-2)。对血吸虫卵(可溶性虫卵抗原和SmTAL2)或体细胞成虫(AHW)抗原的抗体应答在治疗后下降或无变化，而对血吸虫虫抗原(可溶性虫抗原和SmTAL1)的抗体应答增加。观察到的不同治疗效果可能反映了这两种蠕虫不同的药物作用模式和感染部位。重要的是，没有证据表明同时使用吡喹酮和阿苯达唑治疗混合感染的儿童会影响血吸虫和钩虫特异性体液反应，这与只有一种生物是地方性疾病的人群的特征不同；血吸虫和钩虫特异性反应没有关联，也没有交叉调节的证据。治疗后对血吸虫抗原IgE水平的升高与较低的曼氏血吸虫再感染强度有关，而对AHW抗原的体液应答与预防钩虫再感染之间没有相关性。
Naturally occurring human immunity to both schistosomiasis and hookworm infection has been associated with IgE responses against parasite allergen-like proteins. Since the two helminths frequently coinfect the same individuals, there is growing advocacy for their concurrent treatment. However, both helminths are known to exert strong immunomodulatory effects; therefore, coinfected individuals could have immune responses different from those characteristically seen in monoinfected individuals. In this study, we measured changes in IgE, IgG1, and IgG4 responses to schistosome and hookworm antigens, including the allergen-like proteins Schistosoma mansoni tegumental-allergen-like 1 protein (SmTAL1), SmTAL2, and Necator americanus Ancylostoma-secreted protein-2 (Na-ASP-2), following concurrent treatment of schoolchildren coinfected with Schistosoma mansoni and hookworm. Antibody responses to schistosome egg (soluble egg antigen and SmTAL2) or somatic adult hookworm (AHW) antigens either decreased after treatment or were unchanged, whereas those to schistosome worm antigens (soluble worm antigen and SmTAL1) increased. The observed different effects of treatment likely reflect the different modes of drug action and sites of infection for these two helminths. Importantly, there was no evidence that the simultaneous treatment of coinfected children with praziquantel and albendazole affected schistosome-and hookworm-specific humoral responses differently from those characteristic of populations in which only one organism is endemic; schistosome-and hookworm-specific responses were not associated, and there was no evidence for cross-regulation. Posttreatment increases in the levels of IgE to schistosome worm antigens were associated with lower Schistosoma mansoni reinfection intensity, while no associations between humoral responses to AHW antigen and protection from hookworm reinfection were observed in this sample of school-aged children.