NONTOXIC AND DURABLE SALT BRIDGES USING HYDROXYETHYLMETHACRYLATE HYDROGELS
NONTOXIC AND DURABLE SALT BRIDGES USING HYDROXYETHYLMETHACRYLATE HYDROGELS
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DOI：
10.1152/jappl.1990.69.1.373
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发表时间：
1990-07-01
影响因子：
3.3
通讯作者：
BERGETHON, PR
中科院分区：
文献类型：
--
作者：
KINDLER, DD;BERGETHON, PR
Polyhydroxyethylmethacrylate polymers (poly-HEMA) form hydrogels that provide an excellent alternative to agar in the production of salt bridges for use in bioelectrochemical experiments. A method for the simple production of poly-HEMA slat bridges is described. The poly-HEMA bridges were compared with agar bridges of similar geometry. Whereas poly-HEMA salt bridges have a conductivity that is 20 times lower than that of agar bridges of a similar geometry, poly-HEMA bridges are capable of dissipating twice the power compared with agar bridges. The mechanical properties of the poly-HEMA bridges make them easy to manufacture, store, and sterilize. When agar bridges were compared with poly-HEMA bridges in long-term cell culture experiments, the failure rate of the agar bridges was found to be .apprx. 10% per week vs. a virtually nonexistent failure rate for the poly-HEMA bridges. Because poly-HEMA salt bridges are reliable, durable, and nontoxic to cells, they are a practical alternative to agar salt bridges in certain experimental.
