The clinical and prognostic significance of LGR5 in GC: A meta-analysis of IHC assay and bioinformatics analysis
The clinical and prognostic significance of LGR5 in GC: A meta-analysis of IHC assay and bioinformatics analysis
复制标题
DOI：
10.4149/neo_2021_200714n726
复制
发表时间：
2021-01-01
期刊：
影响因子：
3
通讯作者：
Wang, Yu
中科院分区：
文献类型：
--
作者：
Guo, Fei;Yang, Tao;Wang, Yu
富亮氨酸重复序列g蛋白偶联受体5 （leucine-rich repeat-containing g -protein coupled receptor 5, LGR5）是近年来新发现的肿瘤干细胞标志物和Wnt靶基因。然而，LGR5在胃癌（GC）中的作用仍不确定。本研究旨在探讨LGR5表达对胃癌的影响。通过电子数据库检索符合条件的研究。采用95%置信区间（ci）的比值比（ORs）或95% ci的风险比（hr）来估计LGR5的效果。进一步的生物信息学验证数据证实了我们的结果。11项研究纳入2646例胃癌患者。LGR5的表达与年龄、性别、肿瘤分期、T分期、肿瘤大小、淋巴浸润、淋巴结转移和远端转移无关。LGR5表达与肿瘤类型相关（肠型与弥漫性：OR=2.25, p=0.032）。LGR5表达与肿瘤分级呈负相关（3-4级vs. 1-2级：OR = 0.40, p=0.033）。进一步的TCGA验证数据也显示了类似的结果，LGR5的表达也被发现与肿瘤分级呈负相关。多因素Cox分析显示，LGR5表达与总生存期（OS）较差相关（HR=2.54, p=0.009）。进一步的生物信息学数据显示，在876例GCs中，LGR5的表达仍与较短的OS相关。LGR5的表达与肿瘤分级呈负相关，肠型高于弥漫性。此外，LGR5可能是预测胃癌患者生存的潜在预后因素。
Recently, leucine-rich repeat-containing G-protein-coupled receptor 5 (LGR5) is a newly identified cancer stem cell marker and Wnt target gene. However, the role of LGR5 in gastric cancer (GC) remains uncertain. This study was performed to investigate the effect of LGR5 expression in GC. The eligible studies were searched via electronic databases. The odds ratios (ORs) with 95% confidence intervals (CIs) or hazard ratios (HRs) with 95% CIs were applied to estimate the effect of LGR5. Further bioinformatics validation data were used to confirm our results. Eleven studies consisting of 2,646 GC patients were identified. LGR5 expression was not associated with age, gender, tumor stage, T stage, tumor size, lymphatic invasion, lymph node metastasis, and distal metastasis. LGR5 expression was related to tumor type (intestinal vs. diffuse: OR=2.25, p=0.032). LGR5 expression was negatively correlated with tumor grade (grade 3-4 vs. grade 1-2: OR = 0.40, p=0.033). Further TCGA validation data also showed similar findings, and LGR5 expression was also found to have a negative association with tumor grade. LGR5 expression was associated with worse overall survival (OS) using multivariate Cox analysis (HR=2.54, p=0.009). Further bioinformatics data showed that LGR5 expression was still correlated with shorter OS in 876 GCs. LGR5 expression was negatively correlated with tumor grade and its expression was higher in intestinal-type than in diffuse-type. Moreover, LGR5 may be a potential prognostic factor for survival prediction in GC.