N-cadherin differentially determines Schwann cell and olfactory ensheathing cell adhesion and migration responses upon contact with astrocytes
N-cadherin differentially determines Schwann cell and olfactory ensheathing cell adhesion and migration responses upon contact with astrocytes
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DOI：
10.1016/j.mcn.2004.09.009
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发表时间：
2005-02-01
影响因子：
3.5
通讯作者：
Barnett, SC
中科院分区：
文献类型：
--
作者：
Fairless, R;Frame, MC;Barnett, SC
嗅鞘细胞(OECs)和雪旺细胞为多种中枢神经系统损伤模型提供了促进轴突生长的细胞环境。然而，当它们与星形胶质细胞接触时，它们表现出不同的特性。雪旺细胞，但不是嗅鞘细胞，诱导星形胶质细胞肥大的特征，并表现出在星形胶质细胞丰富的区域内迁移的能力较差，使它们不利于移植介导的修复。N-钙粘附素参与了雪旺细胞与星形胶质细胞的黏附。与嗅鞘细胞相比，尽管N-钙粘附素表达不明显，雪旺细胞与星形胶质细胞单层的粘附性更强，迁移速度更慢。我们研究了N-钙粘附素在使用RNA干扰介导这些细胞相互作用中的作用，并发现了不同的效果。在雪旺细胞中，抑制N-钙粘附素减少了异型和同型黏附，它们获得了更类似于嗅鞘细胞的黏附特性。相反，抑制嗅鞘细胞中的N-钙粘附素没有作用。这些发现表明N-钙粘蛋白在嗅鞘细胞和雪旺细胞中受到不同的调控。(C)2004 Elsevier Inc.保留所有权利。
Olfactory ensheathing cells (OECs) and Schwann cells provide a cellular environment that promotes axonal outgrowth in several models of CNS injury. However, they exhibit different properties when in contact with astrocytes. Schwann cells, but not OECs, induce characteristics that typify hypertrophy in astrocytes and exhibit a poor capacity to migrate within astrocyte-rich areas, making them less favourable for transplant-mediated repair. N-cadherin has been implicated in the adhesion of Schwann cells to astrocytes. Despite indistinguishable expression of N-cadherin, Schwann cells adhered more strongly to an astrocyte monolayer and migrated more slowly on astrocytes when compared to OECs. We have examined the role of N-cadherin in mediating these cellular interactions using RNA interference and found differing effects. In Schwann cells, suppression of N-cadherin reduced heterotypic and homotypic adhesion and they gained adhesion properties more akin to OECs. In contrast, suppression of N-cadherin in OECs had no effect. These findings imply that N-cadherin is differentially regulated in OECs and Schwann cells. (C) 2004 Elsevier Inc. All rights reserved.