Neuronal density, size and shape in the human anterior cingulate cortex: a comparison of Nissl and NeuN staining
Neuronal density, size and shape in the human anterior cingulate cortex: a comparison of Nissl and NeuN staining
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DOI：
10.1016/j.brainresbull.2004.02.005
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发表时间：
2004-03-15
影响因子：
3.8
通讯作者：
Harrison, PJ
中科院分区：
文献类型：
--
作者：
Gittins, R;Harrison, PJ
有越来越多的人类大脑皮层的定量形态测量研究，特别是作为主要精神疾病的比较研究的一部分。在这方面，本研究报告有两个目的。首先，提供定量数据的关键神经元形态学参数在前扣带皮层。第二，将常规尼氏染色的结果与用NeuN免疫染色后观察到的结果进行比较，NeuN是一种广泛用作选择性神经元标记物的抗体。我们用甲酚紫或NeuN染色了16个成人脑24 b区的相邻切片。我们测量了锥体和非锥体神经元的密度，以及锥体神经元的大小和形状。Ⅱ、Ⅲ、Ⅴ a、Ⅴ b和Ⅵ层的二维计数。所有变量均观察到两种染色模式之间的强相关性。然而，NeuN给出了略高的神经元密度和大小的估计，以及更圆的核周形状。脑pH值与神经元大小相关，用两种方法测量，并与神经元的形状。年龄和死亡时间与任何参数均无相关性。这些数据证实了NeuN作为常规处理人脑组织中定量神经元形态学研究的工具的价值。NeuN和甲酚紫染色之间的绝对值高度相关，但不能互换。NeuN可能是特别有用的，当它是重要的区分小神经元从神经胶质细胞，如在抑郁症和精神分裂症的大脑皮层的细胞结构研究。(C)2004年爱思唯尔公司All rights reserved.
There are an increasing number of quantitative morphometric studies of the human cerebral cortex, especially as part of comparative investigations of major psychiatric disorders. In this context, the present study had two aims. First, to provide quantitative data regarding key neuronal morphometric parameters in the anterior cingulate cortex. Second, to compare the results of conventional Nissl staining with those observed after immunostaining with NeuN, an antibody becoming widely used as a selective neuronal marker. We stained adjacent sections of area 24b from 16 adult brains with cresyl violet or NeuN. We measured the density of pyramidal and non-pyramidal neurons, and the size and shape of pyramidal neurons. in laminae II, III, Va, Vb and VI, using two-dimensional counting methods. Strong correlations between the two modes of staining were seen for all variables. However, NeuN gave slightly higher estimates of neuronal density and size, and a more circular perikaryal shape. Brain pH was con-elated with neuronal size, measured with both methods, and with neuronal shape. Age and post-mortem interval showed no correlations with any parameter. These data confirm the value of NeuN as a tool for quantitative neuronal morphometric studies in routinely processed human brain tissue. Absolute values are highly correlated between NeuN and cresyl violet stains, but cannot be interchanged. NeuN may be particularly useful when it is important to distinguish small neurons from glia, such as in cytoarchitectural studies of the cerebral cortex in depression and schizophrenia. (C) 2004 Elsevier Inc. All rights reserved.